"
Team:TU Darmstadt/Materials/Chloramphenicol
From 2012.igem.org
(Difference between revisions)
(→Chloramphenicol) |
|||
| (One intermediate revision not shown) | |||
| Line 27: | Line 27: | ||
<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
| - | + | ||
| - | + | ||
| - | + | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
| Line 46: | Line 43: | ||
#* 1 g chloramphenicol (25 mg/mL) | #* 1 g chloramphenicol (25 mg/mL) | ||
#* ad. 40 mL ethanol | #* ad. 40 mL ethanol | ||
| - | + | # aliqout in 1 mL stocks and store at -20°C | |
=== Stability === | === Stability === | ||
Latest revision as of 20:42, 26 September 2012
Contents |
Chloramphenicol
About
Chloramphenicol is a bacteriostatic antibiotic that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.[1]
Stock
- Mix:
- 1 g chloramphenicol (25 mg/mL)
- ad. 40 mL ethanol
- aliqout in 1 mL stocks and store at -20°C
Stability
- Chloramphenicol is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
References
[1] http://en.wikipedia.org/wiki/Chloramphenicol
