Team:Macquarie Australia/Protocols/PlasmidPreparation13thhh

From 2012.igem.org

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Below are the Nano Drop results from the total of 9 cultures which grew. The 'E' stands for electroporated. These total samples underwent plasmid preparation protocol and have been sent to the Macquarie University DNA Sequencing Facility.
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<center>'''NanoDrop Results'''</center>
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{| border="3" cellpadding="4" cellspacing="0" align="center" style="width: 55%; height: 400px"
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|-
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! scope="col"| Culture
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! scope="col"| Concentration (ng/ul)
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! scope="col"| 260/280
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! scope="col"| 260/230
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|-
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| align="center"| 3CE/1
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| align="center"| 43.6
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| align="center"| 1.92
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| align="center"| 2.27
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|-
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| align="center"| 3CE/2
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| align="center"| 38.3
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| align="center"| q.89
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| align="center"| 1.67
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|-
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| align="center"| 3CE/3
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| align="center"| 62.2
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| align="center"| q.91
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| align="center"| 1.77
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|-
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| align="center"| 4KE/1
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| align="center"| 49.5
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| align="center"| 1.82
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| align="center"| 1.23
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|-
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| align="center"| 4KE/2
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| align="center"| 32.3
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| align="center"| 1.78
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| align="center"| 0.91
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|-
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| align="center"| 4KE/3
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| align="center"| 14.4
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| align="center"| 1.89
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| align="center"| 1.75
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|-
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| align="center"| 5CE/1
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| align="center"| 71.1
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| align="center"| 1.76
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| align="center"| 1.08
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|-
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| align="center"| 5CE/2
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| align="center"| 92.1
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| align="center"| 1.76
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| align="center"| 1.17
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|-
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| align="center"| 5CE/3
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| align="center"| 56.7
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| align="center"| 1.86
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| align="center"| 2.08
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|}

Latest revision as of 09:33, 16 September 2012




Below are the Nano Drop results from the total of 9 cultures which grew. The 'E' stands for electroporated. These total samples underwent plasmid preparation protocol and have been sent to the Macquarie University DNA Sequencing Facility.


NanoDrop Results


Culture Concentration (ng/ul) 260/280 260/230


3CE/1 43.6 1.92 2.27


3CE/2 38.3 q.89 1.67
3CE/3 62.2 q.91 1.77


4KE/1 49.5 1.82 1.23
4KE/2 32.3 1.78 0.91
4KE/3 14.4 1.89 1.75
5CE/1 71.1 1.76 1.08
5CE/2 92.1 1.76 1.17
5CE/3 56.7 1.86 2.08
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