Team:Osaka/week7

From 2012.igem.org

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==September 9 (Sun)==
==September 9 (Sun)==
-
# miniprep
+
# Miniprep of yesterday's culture(150,151,152,159,160)
-
# Restriction digests(150,151,152,159,160)
+
# Restriction digests of minipreppped parts
# Electrophoresis
# Electrophoresis
# Tolerance assay of 144,147(MMC)
# Tolerance assay of 144,147(MMC)
-
# To liquid culture(144,145,160)
+
# Transfer to liquid culture(144,145,160)
==September 10 (Mon)==
==September 10 (Mon)==
-
# miniprep
+
# Miniprep of yesterday's culture(160,1-9N)
-
# Restriction digests(160,1-9N)
+
# Restriction digests of minipreppped parts
-
# Electrophoresis
+
# Gel electrophoresis of digests
# Preparation of LB agar plates(Amp)
# Preparation of LB agar plates(Amp)
-
# Ligation(160)
+
# Ligation
-
# Transformation(160)
+
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
-
# To liquid culture(146,148)
+
# Transformation of ligation products into DH5α <i>E.coli</i>
 +
# Transfer to liquid culture(146,148)
# Tolerance assay of 144,145(MMC)
# Tolerance assay of 144,145(MMC)
Line 23: Line 24:
==September 11 (Tue)==
==September 11 (Tue)==
# Restriction digests(157,158,159,160,1-9N)
# Restriction digests(157,158,159,160,1-9N)
-
# Electrophoresis
+
# Gel electrophoresis of digests
-
# Ligation(160)
+
# Ligation
-
# Tolerance assay of 146,148(MMC)
+
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
-
# Transformation(160)
+
# Transformation of ligation products into DH5α <i>E.coli</i>
-
 
+
# Tolerance assay of 146,148(MMC)  
==September 12 (Wed)==
==September 12 (Wed)==
-
# miniprep
+
# Miniprep of yesterday's culture:160
-
# Restriction digests(160)
+
# Restriction digests of minipreppped parts
-
# Electrophoresis
+
# Gel electrophoresis of digests
# Tolerance assay
# Tolerance assay
-
# Ligation(161,162)
+
# Ligation
-
 
+
#* 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
 +
#* 162(C):159(upstream)+160(downstream)+1-3A(vector)
 +
# Transformation of ligation products into DH5α <i>E.coli</i>
==September 13 (Thu)==
==September 13 (Thu)==
 +
# Colony check
 +
#* 161:Transformed cells didnot grow
# Sequencing(157,158,159,160)
# Sequencing(157,158,159,160)
# Tolerance assay of 146,148
# Tolerance assay of 146,148
-
# Transformation(161)
+
# Transformation of ligation products into DH5α <i>E.coli</i>:161
-
# To liquid culture(144~148,162)
+
# Transfer to liquid culture(144~148,162)
==September 14 (Fri)==
==September 14 (Fri)==
-
# miniprep
+
# Miniprep of yesterday's culture:162
-
# Restriction digests(162)
+
# Restriction digests of minipreppped parts
# Tolerance assay (Preliminary test of hydrogen peroxide)
# Tolerance assay (Preliminary test of hydrogen peroxide)
-
# Electrophoresis(162)
+
# Gel electrophoresis of digests
-
# To liquid culture(161)
+
# Transfer to liquid culture:149,152,161
==September 15 (Sat)==
==September 15 (Sat)==
-
 
+
# Miniprep of yesterday's culture:161
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
# Transformation of ligation products into DH5α <i>E.coli</i>:161
 +
# Tolerance assay (Hydrogen peroxide)
 +
# Transfer to liquid culture:149,150,151,161
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]

Latest revision as of 07:46, 15 September 2012


September 9 (Sun)

  1. Miniprep of yesterday's culture(150,151,152,159,160)
  2. Restriction digests of minipreppped parts
  3. Electrophoresis
  4. Tolerance assay of 144,147(MMC)
  5. Transfer to liquid culture(144,145,160)


September 10 (Mon)

  1. Miniprep of yesterday's culture(160,1-9N)
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Preparation of LB agar plates(Amp)
  5. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  6. Transformation of ligation products into DH5α E.coli
  7. Transfer to liquid culture(146,148)
  8. Tolerance assay of 144,145(MMC)


September 11 (Tue)

  1. Restriction digests(157,158,159,160,1-9N)
  2. Gel electrophoresis of digests
  3. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  4. Transformation of ligation products into DH5α E.coli
  5. Tolerance assay of 146,148(MMC)

September 12 (Wed)

  1. Miniprep of yesterday's culture:160
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Tolerance assay
  5. Ligation
    • 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
    • 162(C):159(upstream)+160(downstream)+1-3A(vector)
  6. Transformation of ligation products into DH5α E.coli

September 13 (Thu)

  1. Colony check
    • 161:Transformed cells didnot grow
  2. Sequencing(157,158,159,160)
  3. Tolerance assay of 146,148
  4. Transformation of ligation products into DH5α E.coli:161
  5. Transfer to liquid culture(144~148,162)


September 14 (Fri)

  1. Miniprep of yesterday's culture:162
  2. Restriction digests of minipreppped parts
  3. Tolerance assay (Preliminary test of hydrogen peroxide)
  4. Gel electrophoresis of digests
  5. Transfer to liquid culture:149,152,161

September 15 (Sat)

  1. Miniprep of yesterday's culture:161
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformation of ligation products into DH5α E.coli:161
  5. Tolerance assay (Hydrogen peroxide)
  6. Transfer to liquid culture:149,150,151,161


Back to Notebook

Retrieved from "http://2012.igem.org/Team:Osaka/week7"