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Team:Osaka/week7
From 2012.igem.org
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==September 9 (Sun)== | ==September 9 (Sun)== | ||
| - | + | # Miniprep of yesterday's culture(150,151,152,159,160) | |
| + | # Restriction digests of minipreppped parts | ||
| + | # Electrophoresis | ||
| + | # Tolerance assay of 144,147(MMC) | ||
| + | # Transfer to liquid culture(144,145,160) | ||
==September 10 (Mon)== | ==September 10 (Mon)== | ||
| - | + | # Miniprep of yesterday's culture(160,1-9N) | |
| + | # Restriction digests of minipreppped parts | ||
| + | # Gel electrophoresis of digests | ||
| + | # Preparation of LB agar plates(Amp) | ||
| + | # Ligation | ||
| + | #* 160(K):157(upstream)+158(downstream)+1-5A(vector) | ||
| + | # Transformation of ligation products into DH5α <i>E.coli</i> | ||
| + | # Transfer to liquid culture(146,148) | ||
| + | # Tolerance assay of 144,145(MMC) | ||
==September 11 (Tue)== | ==September 11 (Tue)== | ||
| - | + | # Restriction digests(157,158,159,160,1-9N) | |
| - | + | # Gel electrophoresis of digests | |
| + | # Ligation | ||
| + | #* 160(K):157(upstream)+158(downstream)+1-5A(vector) | ||
| + | # Transformation of ligation products into DH5α <i>E.coli</i> | ||
| + | # Tolerance assay of 146,148(MMC) | ||
==September 12 (Wed)== | ==September 12 (Wed)== | ||
| - | + | # Miniprep of yesterday's culture:160 | |
| - | + | # Restriction digests of minipreppped parts | |
| + | # Gel electrophoresis of digests | ||
| + | # Tolerance assay | ||
| + | # Ligation | ||
| + | #* 161(C):1-9N(upstream)+160(downstream)+1-3A(vector) | ||
| + | #* 162(C):159(upstream)+160(downstream)+1-3A(vector) | ||
| + | # Transformation of ligation products into DH5α <i>E.coli</i> | ||
==September 13 (Thu)== | ==September 13 (Thu)== | ||
| - | + | # Colony check | |
| + | #* 161:Transformed cells didnot grow | ||
| + | # Sequencing(157,158,159,160) | ||
| + | # Tolerance assay of 146,148 | ||
| + | # Transformation of ligation products into DH5α <i>E.coli</i>:161 | ||
| + | # Transfer to liquid culture(144~148,162) | ||
==September 14 (Fri)== | ==September 14 (Fri)== | ||
| - | + | # Miniprep of yesterday's culture:162 | |
| - | + | # Restriction digests of minipreppped parts | |
| + | # Tolerance assay (Preliminary test of hydrogen peroxide) | ||
| + | # Gel electrophoresis of digests | ||
| + | # Transfer to liquid culture:149,152,161 | ||
==September 15 (Sat)== | ==September 15 (Sat)== | ||
| - | + | # Miniprep of yesterday's culture:161 | |
| + | # Restriction digests of minipreppped parts | ||
| + | # Gel electrophoresis of digests | ||
| + | # Transformation of ligation products into DH5α <i>E.coli</i>:161 | ||
| + | # Tolerance assay (Hydrogen peroxide) | ||
| + | # Transfer to liquid culture:149,150,151,161 | ||
[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] | ||
Latest revision as of 07:46, 15 September 2012
September 9 (Sun)
- Miniprep of yesterday's culture(150,151,152,159,160)
- Restriction digests of minipreppped parts
- Electrophoresis
- Tolerance assay of 144,147(MMC)
- Transfer to liquid culture(144,145,160)
September 10 (Mon)
- Miniprep of yesterday's culture(160,1-9N)
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Preparation of LB agar plates(Amp)
- Ligation
- 160(K):157(upstream)+158(downstream)+1-5A(vector)
- Transformation of ligation products into DH5α E.coli
- Transfer to liquid culture(146,148)
- Tolerance assay of 144,145(MMC)
September 11 (Tue)
- Restriction digests(157,158,159,160,1-9N)
- Gel electrophoresis of digests
- Ligation
- 160(K):157(upstream)+158(downstream)+1-5A(vector)
- Transformation of ligation products into DH5α E.coli
- Tolerance assay of 146,148(MMC)
September 12 (Wed)
- Miniprep of yesterday's culture:160
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Tolerance assay
- Ligation
- 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
- 162(C):159(upstream)+160(downstream)+1-3A(vector)
- Transformation of ligation products into DH5α E.coli
September 13 (Thu)
- Colony check
- 161:Transformed cells didnot grow
- Sequencing(157,158,159,160)
- Tolerance assay of 146,148
- Transformation of ligation products into DH5α E.coli:161
- Transfer to liquid culture(144~148,162)
September 14 (Fri)
- Miniprep of yesterday's culture:162
- Restriction digests of minipreppped parts
- Tolerance assay (Preliminary test of hydrogen peroxide)
- Gel electrophoresis of digests
- Transfer to liquid culture:149,152,161
September 15 (Sat)
- Miniprep of yesterday's culture:161
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformation of ligation products into DH5α E.coli:161
- Tolerance assay (Hydrogen peroxide)
- Transfer to liquid culture:149,150,151,161
