Team:TU Darmstadt/Materials/tctB162

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Line 46: Line 43:
  rev: gtttcttc'''ctgcagcggccgctactagta'''ttagccagcgatgaagctaggcc
  rev: gtttcttc'''ctgcagcggccgctactagta'''ttagccagcgatgaagctaggcc
=== tctB162 Pst1 ===
=== tctB162 Pst1 ===
-
[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for removing a Pst1 site.
+
[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the elimination of a [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI Pst1] site inside the tctB162 BioBrick.
       5'                            3'
       5'                            3'
  fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
  fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
  rev: GGCCACACGGGGAACTGGAGATTCAGCGCC
  rev: GGCCACACGGGGAACTGGAGATTCAGCGCC
 +
 +
=== tctB162 RBS ===
 +
[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the insertion of a RBS based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101].
 +
      5'                                                                                3'
 +
fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAAAGAGGGGACAAtactag atgaaaatcaagagccaaaaagactttttctc
 +
rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttagccagcgatgaagctaggcc

Latest revision as of 20:50, 26 September 2012

Contents

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB162]

Primers for the construction of a BioBrick containing the small subunit B1 of the tripartite tricarboxylate transporter family.

tctB162 BioBrick 

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgaaaatcaagagccaaaaagactttttctc
rev: gtttcttcctgcagcggccgctactagtattagccagcgatgaagctaggcc

tctB162 Pst1

Primers for the elimination of a Pst1 site inside the tctB162 BioBrick. 

     5'                             3'
fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
rev: GGCCACACGGGGAACTGGAGATTCAGCGCC

tctB162 RBS

Primers for the insertion of a RBS based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101]. 

     5'                                                                                 3'
fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAAAGAGGGGACAAtactag atgaaaatcaagagccaaaaagactttttctc
rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttagccagcgatgaagctaggcc
Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162"