Team:TU Darmstadt/Materials/tctA505

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=== tctA505 Pst1 ===
=== tctA505 Pst1 ===
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[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for elimination of a Pst1 site.
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[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for elimination of a [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI Pst1] site.
       5'                            3'
       5'                            3'
  fwd: CTGGTGGATCTTCCTTCAGCGCCCCATGTCG
  fwd: CTGGTGGATCTTCCTTCAGCGCCCCATGTCG
  rev: CGACATGGGGCGCTGAAGGAAGATCCACCAG
  rev: CGACATGGGGCGCTGAAGGAAGATCCACCAG

Latest revision as of 20:51, 26 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808004 tctA505]

Primers for the construction of a BioBrick large subunit A2 of the tripartite tricarboxylate transporter family.

tctA505 BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatggatattttcaatgctttgatggctgg
rev: gtttcttcctgcagcggccgctactagtatcaggcggtgcttgcctccc

tctA505 Pst1

Primers for elimination of a Pst1 site.

     5'                             3'
fwd: CTGGTGGATCTTCCTTCAGCGCCCCATGTCG
rev: CGACATGGGGCGCTGAAGGAAGATCCACCAG