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Team:TU Darmstadt/Materials/Coomassie stain
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<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
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== Coomassie stain == | == Coomassie stain == | ||
=== About === | === About === | ||
| - | Coomassie Brilliant Blue is the name of a two component triphenylmethane dye commonly used for staining proteins. | + | Coomassie Brilliant Blue is the name of a two component triphenylmethane dye commonly used for staining proteins in [https://2012.igem.org/Team:TU_Darmstadt/Materials#PAGE SDS page]. |
=== Ingredients === | === Ingredients === | ||
Mix together the following contents under a fume hood: | Mix together the following contents under a fume hood: | ||
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* 430mL dH2O | * 430mL dH2O | ||
* 1g Brilliant Blue | * 1g Brilliant Blue | ||
| + | === Application === | ||
| + | * SDS page gel is placed in a small tub and covered in stain | ||
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Materials/Destain Destaining] is accomplished with a mix of methanol and acetic acid | ||
Latest revision as of 20:55, 26 September 2012
Contents |
Coomassie stain
About
Coomassie Brilliant Blue is the name of a two component triphenylmethane dye commonly used for staining proteins in SDS page.
Ingredients
Mix together the following contents under a fume hood:
- 0.5L Methanol
- 70mL Acetic Acid
- 430mL dH2O
- 1g Brilliant Blue
Application
- SDS page gel is placed in a small tub and covered in stain
- Destaining is accomplished with a mix of methanol and acetic acid
