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Team:TU Darmstadt/Materials/Ethidium Bromide
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Ethidium bromide aka EtBr is a marker for nucleic acids in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis agarose gel electrophoresis]. It does intercalate between DNA base pairs and fluorescents under UV light. | Ethidium bromide aka EtBr is a marker for nucleic acids in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis agarose gel electrophoresis]. It does intercalate between DNA base pairs and fluorescents under UV light. | ||
=== Application === | === Application === | ||
| + | For DNA staining the agarose gel is mixed with 0.0005 vol % of ethidium bromide solution (1mg/µl). | ||
| + | For more information take a look at the [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gele electrophoresis protocol]. | ||
=== Notes === | === Notes === | ||
'''CAUTION:''' EtBr is carcinogen and mutagen, the penetration time of EtBr on latex gloves is less than 5s, nitrile gloves must be worn!<sup>[1]</sup> | '''CAUTION:''' EtBr is carcinogen and mutagen, the penetration time of EtBr on latex gloves is less than 5s, nitrile gloves must be worn!<sup>[1]</sup> | ||
| + | |||
| + | [https://2012.igem.org/Team:TU_Darmstadt/Materials/Safe_stain Roti<sup>®</sup> SafeGelStain] is a less toxic alternative. | ||
Due to its hazardous properties EtBr must be handled and disposed accordingly. | Due to its hazardous properties EtBr must be handled and disposed accordingly. | ||
Latest revision as of 20:53, 26 September 2012
Contents |
Ethidium bromide
About
Ethidium bromide aka EtBr is a marker for nucleic acids in agarose gel electrophoresis. It does intercalate between DNA base pairs and fluorescents under UV light.
Application
For DNA staining the agarose gel is mixed with 0.0005 vol % of ethidium bromide solution (1mg/µl).
For more information take a look at the Agarose gele electrophoresis protocol.
Notes
CAUTION: EtBr is carcinogen and mutagen, the penetration time of EtBr on latex gloves is less than 5s, nitrile gloves must be worn![1]
Roti® SafeGelStain is a less toxic alternative.
Due to its hazardous properties EtBr must be handled and disposed accordingly.
Supplier
...
References
[1] http://www.newmarketscientific.com/datasheets/Novel_Juice_Testing_Report_012011.pdf
