"
Team:TU Darmstadt/Materials/Bromphenol blue
From 2012.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 27: | Line 27: | ||
<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
| - | + | ||
| - | + | ||
| - | + | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
| Line 41: | Line 38: | ||
== Bromphenol blue == | == Bromphenol blue == | ||
=== About === | === About === | ||
| - | Bromphenol blue is a size marker used in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis agarose gel electrophoresis] | + | Bromphenol blue is a size marker used in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis agarose gel electrophoresis]<sup>[1]</sup> that does also work as a pH indicator. Below pH=3.0 it is yellow and blue-purple at more basic pH. Its specific band depends on the gel concentration:<sup>[2]</sup> |
| - | Its specific band depends on the gel concentration:<sup>[2]</sup> | + | |
{| class="wikitable sortable" | {| class="wikitable sortable" | ||
|- | |- | ||
| Line 54: | Line 50: | ||
|- | |- | ||
|style="text-align:center"| 3 || 50 | |style="text-align:center"| 3 || 50 | ||
| - | |} | + | |} |
=== Application === | === Application === | ||
Latest revision as of 20:52, 26 September 2012
Contents |
Bromphenol blue
About
Bromphenol blue is a size marker used in agarose gel electrophoresis[1] that does also work as a pH indicator. Below pH=3.0 it is yellow and blue-purple at more basic pH. Its specific band depends on the gel concentration:[2]
| % Agarose | bp |
|---|---|
| 0,7 | 1000 |
| 1 | 500 |
| 2 | 150 |
| 3 | 50 |
Application
References
[1] http://en.wikipedia.org/wiki/Bromophenol_blue
[2] http://openwetware.org/wiki/Bromophenol_blue
