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Team:TU Darmstadt/Materials/Agarose gel loading buffer
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Latest revision as of 20:51, 26 September 2012
Contents |
Agarose gel loading buffer
About
Loading buffer also known as loading dye is mixed with DNA samples for use in agarose gel electrophoresis. It contains a dye to assess the progress of the electrolysis and reagents that render the samples denser than the running buffer (so that the samples sink in the well).[1]
Application
Loading dye usually comes in 5X concentration. To use, add and mix 1/5th volume dye/sample (in order to save material mixing on [http://en.wikipedia.org/wiki/Parafilm Parafilm] is an option). It is crucial that the dye and the expected DNA size do not overlap. Take a look in the spec sheet provided with the stain.
Supplier
...
References
[1] http://openwetware.org/wiki/Agarose_gel_loading_buffer
