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Team:TU Darmstadt/Materials/Phusion
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Latest revision as of 20:44, 26 September 2012
Contents |
Phusion
About
Phusion is a Pyrococcus Pfu-like DNA polymerase with a dobule-strand DNA binding domain, resulting in an enhanced processivity and higher activity than other available DNA polymerases like taq and creates blunt ends.
Working conditions
- 98°C denaturation temperature
- ~30 s/kb extension velocity (at 72°C)
- (TM-3)°C=TA
References
- http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf
