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Team:TU Darmstadt/Materials/Chloramphenicol
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== Chloramphenicol == | == Chloramphenicol == | ||
=== About === | === About === | ||
| - | Chloramphenicol is a bacteriostatic [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.[1] | + | Chloramphenicol is a bacteriostatic [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.<sup>[1]</sup> |
| + | === Stock === | ||
| + | # Mix: | ||
| + | #* 1 g chloramphenicol (25 mg/mL) | ||
| + | #* ad. 40 mL ethanol | ||
| + | # aliqout in 1 mL stocks and store at -20°C | ||
=== Stability === | === Stability === | ||
| - | * Chloramphenicol is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it) | + | * Chloramphenicol is not autoclavable, it has to be added before [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Pouring_LB_plates pouring plates] (keep in mind that the agar is not too hot when adding it) |
=== References === | === References === | ||
| - | + | [1] http://en.wikipedia.org/wiki/Chloramphenicol | |
Latest revision as of 20:42, 26 September 2012
Contents |
Chloramphenicol
About
Chloramphenicol is a bacteriostatic antibiotic that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.[1]
Stock
- Mix:
- 1 g chloramphenicol (25 mg/mL)
- ad. 40 mL ethanol
- aliqout in 1 mL stocks and store at -20°C
Stability
- Chloramphenicol is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
References
[1] http://en.wikipedia.org/wiki/Chloramphenicol
