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Team:TU Darmstadt/Materials/SOB
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=== SOB === | === SOB === | ||
| - | The SOB medium is used for growth of bacteria | + | The SOB medium is used for growth of bacteria in preparation of [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically competent cells]. |
==== Ingredients ==== | ==== Ingredients ==== | ||
Latest revision as of 20:41, 26 September 2012
SOB
The SOB medium is used for growth of bacteria in preparation of chemically competent cells.
Ingredients
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
For 1 L:
- 0.186 g KCl
- 2.4 g MgSO4
- 0.584 g NaCl
- 20 g tryptone
- 5 g yeast extract
Adjust to pH 7.5 prior to use. This requires approximately 5 mL of 5M NaOH per liter.
