Team:Ehime-Japan

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<p  style="text-align:center;"><a href="https://2012.igem.org/Team:Ehime-Japan"><img src="https://static.igem.org/mediawiki/2012/f/fa/Igem-ehime-001.gif" width="900px"></a></p>
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<p  style="text-align:center;"><a href="https://2012.igem.org/Team:Ehime-Japan"><img src="https://static.igem.org/mediawiki/2012/b/b4/Ecolingual%E3%82%A6%E3%82%A3%E3%82%AD%E7%94%A8%EF%BC%92.png" width="800px"></a></p>
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<ul id="nav">
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        <li><a href="https://2012.igem.org/Team:Ehime-Japan">Home</a></li> 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Project">Project</a>
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<ul>
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#one">E.co-mail</a></li>
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#two">E.co-Domino</a></li>
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                        <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#three">E.cold-heat sensing system</a></li>
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#four">E.colingual</a></li>
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</ul>
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</li>
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        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Modeling">Modeling</a></li>
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<li><a href="#">Data</a>
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<ul>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Parts">BioBrick parts</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/PlasmidsAndStrain">Plasmids And Strain</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/PrimerList">Primer List</a></li>
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</ul>
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</li>
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<li><a href="#">Human Practice</a>
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<ul>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create">E.create</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire">Questionnaire</a></li>
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</ul>
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</li>
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<li><a href="#">Other</a>
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<ul>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Team">team</a></li>
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<li><a href="https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan">team profile</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Notebook">note book</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Safety">safety</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Attributions">attribution</a></li> 
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Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know <i>E. coli</i> feelings. It consists of three parts: Main, Connection, and Screen.<br>
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To realize our project, we used the light sensor genes from <i>Synechocystis</i>, the Lon protease and the <i>ssrA</i> tag from <i>Mesoplasma florum</i>. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.
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<p>
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“E.co-mail” is a communication tool with <i>E. coli</i> and an optical fiber. We would be able to transfer digital information depicted in <i>E.coli</i> through a distance, and probably could send e-mails with this tool. 
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</p>
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a>
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<img src="https://static.igem.org/mediawiki/2012/2/2d/Ecodomino_ehime_un.png" width=800px>
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<font size="3">
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<p><br><br>
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<img src="https://static.igem.org/mediawiki/2012/9/9d/Eco-Domino.png" width=700px><br><br>
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    We tried to reconstruct toppling dominoes with <i>E. coli</i>,
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    and we would be able to draw a movie of firework on LB plate!
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<br><img src="https://static.igem.org/mediawiki/2012/f/f4/%E2%91%A6.png"width="200px">
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</p> 
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#two"><p><font color=blue>See more…</font color><p></a>
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<font size="5">Overview</font>
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  Ehime-Japan team aims to create the communication systems which are circulatable. To realize our projects, we use cyanobacterial genes, Lux and Las systems, Lon protease and ssrA from mycoplasma. Green light sensor and red light sensor genes from Synechocystis are used as "Cell-cell signaling" like Lux and Las systems. To make a circular system, we develop a degradation system with Lon protease and ssrA from Mesoplasma florum.
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<p>
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<BR>
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    Main is comprised of two different kinds of <i>E. coli</i> transformants.
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    One is that feels heat and cold and respond differently, producing different
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    signaling molecules. The other catches the signaling molecules and convert
 +
    the signal into the form of light. As a result, we can see a temperature change
 +
    around the <i>E. coli</i>. The light signals could be transferred through a distance
 +
    and be displayed if the Connection and Screen systems work.
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</p>
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<br>
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#three"><p><font color=blue>See more…</font color><p></a>
<BR>
<BR>
<BR>
<BR>
<BR>
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<font size="5">Background</font>
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<font size="5">
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<img src="https://static.igem.org/mediawiki/2012/0/01/Ecolingual.png" width=800px>
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<br>
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<br>
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<font size="3">
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<p><br><br>
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<img src="
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https://static.igem.org/mediawiki/2012/9/93/E.colingual.png" width=800px><br><br>
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    It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which
 +
    we can know <i>E. coli</i> feelings:“hot”,“cold”, “happy” ,or “dying” etc…
 +
    Also, by an optical fiber, the information of those feelings is transmitted
 +
    to someone in a distant place from a lab. Actually, we focused on <i>E. coli</i>
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    feelings, “hot” and “cold”.
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</p>
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<br>
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<a href=""><p><font color=blue>See more…</font color><p></a>
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<br>
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<br>
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<br>
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<hr>
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<br>
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<br>
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<font size="5">
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→  New degradation system
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<br>
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<br>
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<font size="3">
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<p>
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    We used two different <i>ssrA</i> tags to make a circulatable communication system. One is from
 +
    <i>M. florum</i> recognized by the Lon protease from <i>M. florum</i>. The other is <i>E. coli ssrA</i> tag (LVA).
 +
    Because the sequences of those tags are not similar, the degradation systems should function
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    independently.     
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</p>
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<br>
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a>
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<br>
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<br>
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<br><hr>
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<br>
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<br>
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<font size="5"> 
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→ New parts
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<br>
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<br>
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<font size="3">
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<p>
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    We made new BioBrick parts!
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<br>
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  ・BBa_K794000 : This part is cold shock promoter + <i>lasI</i>.
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</p>
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<br>
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<a href="https://2012.igem.org/Team:Ehime-Japan/Parts"><p><font color=blue>See more…</font color><p></a>
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<br>
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<br>
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<br><hr>
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<br>
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<font size="5">
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→ Human Practice
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<br>
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<br>
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<font size="3">
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<p>
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Ehime-Japan did a lot of projects as Human Practice!
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</p>
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<br>
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<br>
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<font size="5">
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・Ecreate !
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<br>
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<br>
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<br>
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<font size="3">
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<p>
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    We are enjoying gene recombination, and to tell interests of synthetic biology for
 +
    high school students, we made “E.create”!! It is a card game that people can enjoy
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    building genetic circuits. Also, we did educational activities for high school students
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    and played them with E.create! 
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</p>
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<br>     
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<a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create"><p><font color=blue>See more…</font color><p></a>
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<br>
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<font size="5">
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・Questionnaire
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<br>
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<br>
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<font size="3">
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<p>
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    Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together!
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    Thus, we were able to obtain a lot of replies, and found more general opinions
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    about synthetic biology in Japan.
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</p>
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<br>
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<a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire"><p><font color=blue>See more…</font color><p></a>
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<br>
<HR size="10" width="100%" align="left">
<HR size="10" width="100%" align="left">
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  We thought that it sounds fun if a communication system is built with E. coli and it is used repeatedly. In addition, today, it is very important to recycle what someone used. So, we chose our projects.
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Latest revision as of 03:52, 27 September 2012

Ehime-Japan iGEM Team: Welcome









Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know E. coli feelings. It consists of three parts: Main, Connection, and Screen.
To realize our project, we used the light sensor genes from Synechocystis, the Lon protease and the ssrA tag from Mesoplasma florum. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.








“E.co-mail” is a communication tool with E. coli and an optical fiber. We would be able to transfer digital information depicted in E.coli through a distance, and probably could send e-mails with this tool.


See more…










We tried to reconstruct toppling dominoes with E. coli, and we would be able to draw a movie of firework on LB plate!


See more…






Main is comprised of two different kinds of E. coli transformants. One is that feels heat and cold and respond differently, producing different signaling molecules. The other catches the signaling molecules and convert the signal into the form of light. As a result, we can see a temperature change around the E. coli. The light signals could be transferred through a distance and be displayed if the Connection and Screen systems work.


See more…










It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which we can know E. coli feelings:“hot”,“cold”, “happy” ,or “dying” etc… Also, by an optical fiber, the information of those feelings is transmitted to someone in a distant place from a lab. Actually, we focused on E. coli feelings, “hot” and “cold”.


See more…






→ New degradation system

We used two different ssrA tags to make a circulatable communication system. One is from M. florum recognized by the Lon protease from M. florum. The other is E. coli ssrA tag (LVA). Because the sequences of those tags are not similar, the degradation systems should function independently.


See more…






→ New parts

We made new BioBrick parts!
・BBa_K794000 : This part is cold shock promoter + lasI.


See more…






→ Human Practice

Ehime-Japan did a lot of projects as Human Practice!



・Ecreate !


We are enjoying gene recombination, and to tell interests of synthetic biology for high school students, we made “E.create”!! It is a card game that people can enjoy building genetic circuits. Also, we did educational activities for high school students and played them with E.create!


See more…


・Questionnaire

Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together! Thus, we were able to obtain a lot of replies, and found more general opinions about synthetic biology in Japan.


See more…









Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions
Retrieved from "http://2012.igem.org/Team:Ehime-Japan"