"
Team:Wageningen UR/Journal/week6
From 2012.igem.org
MarkvanVeen (Talk | contribs) (→lab work) |
(→lab work) |
||
| Line 15: | Line 15: | ||
CCMV Bricking: | CCMV Bricking: | ||
| - | The | + | The monday morning of this week started with a bit of a shock: our plates with our transformed ''E.coli'' we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed ''E.coli'', we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks. |
CCMV test protocol: | CCMV test protocol: | ||
| - | We've also made | + | We've also made two samples of wt CCMV VLP ready for dialysis. There is only one modification applied in the protocol, instead of using the horrible sonicator, we used the French press, which is a great apparatus and easy to use. In the late afternoon we started with the dialysis. |
written by: Mark | written by: Mark | ||
Revision as of 08:08, 5 June 2012
week 5: 4 june - 8 june
office work
This monday we've worked further on updating the wiki, we are now on scedule with the notebook and protocols. We also made a master plan on how the wiki should look like. The first steps are being made to create a prize winning wiki page.
[Meeting]
written by: Mark
lab work
CCMV Bricking:
The monday morning of this week started with a bit of a shock: our plates with our transformed E.coli we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed E.coli, we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks.
CCMV test protocol:
We've also made two samples of wt CCMV VLP ready for dialysis. There is only one modification applied in the protocol, instead of using the horrible sonicator, we used the French press, which is a great apparatus and easy to use. In the late afternoon we started with the dialysis.
written by: Mark
