Team:Wageningen UR/Journal/week6

From 2012.igem.org

(Difference between revisions)
(lab work)
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== lab work ==
== lab work ==
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 +
Monday:
Bricks:
Bricks:
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The early monday of this week started with a bit of a shock, our plates with our transformed ''E.coli'' we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed ''E.coli'', we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks.
The early monday of this week started with a bit of a shock, our plates with our transformed ''E.coli'' we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed ''E.coli'', we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks.
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CCMV test protocol:
CCMV test protocol:
 +
We've also made different samples ready for dialisis. There is one modification used in the protocol, instead of using the horrible sonifier, we used the French press, which is a great apperatus and easy to use. In the late afternoon we started with the dialysis.
We've also made different samples ready for dialisis. There is one modification used in the protocol, instead of using the horrible sonifier, we used the French press, which is a great apperatus and easy to use. In the late afternoon we started with the dialysis.

Revision as of 06:58, 5 June 2012


week 5: 4 june - 8 june

office work

This monday we've worked further on updating the wiki, we are now on scedule with the notebook and protocols. We also made a master plan on how the wiki should look like. The first steps are being made to create a prize winning wiki page.

[Meeting]

written by: Mark

lab work

Monday:

Bricks:

The early monday of this week started with a bit of a shock, our plates with our transformed E.coli we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed E.coli, we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks.


CCMV test protocol:

We've also made different samples ready for dialisis. There is one modification used in the protocol, instead of using the horrible sonifier, we used the French press, which is a great apperatus and easy to use. In the late afternoon we started with the dialysis.