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Team:Osaka/week5
From 2012.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
# Preparation of LB agar plates (Amp,Cam) | # Preparation of LB agar plates (Amp,Cam) | ||
# Ligation(155,156) | # Ligation(155,156) | ||
| - | # | + | # Transformaton of ligation products into DH5α <i>E.coli</i>:155,156,152(positive control) |
| - | # To liquid culture(152,155, | + | # To liquid culture(152,155,Rosetta wild type) |
==August 29 (Wed)== | ==August 29 (Wed)== | ||
Revision as of 13:36, 28 August 2012
August 26 (Sun)
- Colony check
- 1-1G,1-5A → Successfully transformed
- 5-23O,1-7A → Transformed cells didn't grow on plates
- Transfer to liquid culture:1-1G,1-5A
- Preparation of LB agar plates (Tet)
August 27 (Mon)
- miniprep(1-1G,1-5A)
- Transformation(155,156)
- Transformed cells did not grow on plates
August 28 (Tue)
- Preparation of LB agar plates (Amp,Cam)
- Ligation(155,156)
- Transformaton of ligation products into DH5α E.coli:155,156,152(positive control)
- To liquid culture(152,155,Rosetta wild type)
