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Team:Wageningen UR/Journal/week14
From 2012.igem.org
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the digestion check confirmed the right insert sizes in the samples they where expected (slots 3,4,10,11) | the digestion check confirmed the right insert sizes in the samples they where expected (slots 3,4,10,11) | ||
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| + | '''TuYV''' | ||
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| + | * The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 ''E coli''. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps! | ||
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[[https://2012.igem.org/Team:Wageningen_UR/Journal/week13 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week15 next week]] | [[https://2012.igem.org/Team:Wageningen_UR/Journal/week13 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week15 next week]] | ||
Revision as of 13:04, 18 August 2012
week 14: 30 july - 5 august
Lab work
Bricking Hepatitis B
Monday:
- Miniprepping
of 8 colonies from the transformation (Hep B + BBa_J04500)
- Digestion check
of those minipreps
Wednesday:
- Second digestion check of the minipreps (with higher amount of DNA)
the digestion check confirmed the right insert sizes in the samples they where expected (slots 3,4,10,11)
TuYV
- The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 E coli. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps!
