Team:WashU/Protocols

From 2012.igem.org

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<div id = "protocol">
<font face = "Palatino Linotype, Book Antiqua">
<font face = "Palatino Linotype, Book Antiqua">
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<div align="center">
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=<u>Protocols</u>=
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<div align = "left">
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=General Protocols=
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=='''Week 1'''==
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[https://2012.igem.org/Team:WashU/Protocols/gel_electrophoresis General Agarose Gel Electrophoresis]
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== Protocols ==
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<font size="3.2">''Synechocystis'' Cloning
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<br>
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<font size="2.8">
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[http://www.methodbook.net/dna/agarogel.html Agarose gel electrophoresis]
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[https://2012.igem.org/Team:WashU/Protocols/PCR General PCR Protocol]
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[https://2012.igem.org/Team:WashU/Protocols/Digestion General Digestion Protocol]
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=='''Week 2'''==
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[https://2012.igem.org/Team:WashU/Protocols/LargeDoubleDigestion#Large_Scale_Double_Digest Large Scale Double Digest]
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==Protocols==
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[https://docs.google.com/viewer?a=v&q=cache:TRFtg7R9cokJ:ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf+&hl=en&gl=us&pid=bl&srcid=ADGEESi7CDB7jR-6l_5xh7WoHpGedD6xRfDrte2NZZ-neeODUmAH-JijmNdPq5nr6jZFxavNqTqxOGzr91xFwpsOySod6vvXtnct8aZmM7RvfgX2B-fOJXYir8Ww0jYxPYV504tw6_c9&sig=AHIEtbSx8ChQiPiqqyf9AKDoQmSlGkpWFg&pli=1 Biobrick Assembly]
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[https://2012.igem.org/Team:WashU/Protocols/Ligation General Ligation Protocol]
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=='''Week 3'''==
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[https://2012.igem.org/Team:WashU/Protocols/Transformation General Transformation Protocol]
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== Protocols ==
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[https://2012.igem.org/Team:WashU/Protocols/GelPurification General Gel Purification Protocol]
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[https://2012.igem.org/Team:WashU/Protocols/LBPlates LB Plates (*includes instruction for liquid media)]
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[https://2012.igem.org/Team:WashU/Protocols/GlycerolStock Glycerol Stocks]
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[https://2012.igem.org/Team:WashU/Protocols/SDSPAGE SDS-PAGE gel electrophoresis]
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[https://2012.igem.org/Team:WashU/Protocols/SDSPAGE15 15% SDS-PAGE gel making protocol]
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[https://2012.igem.org/Team:WashU/Protocols/Commassie Coomassie Staining of Acrylamide gels]
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[https://2012.igem.org/Team:WashU/Protocols/Celllysis Cell Lysis and Protein Purification Prep]
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[https://2012.igem.org/Team:WashU/Protocols/Trypsin Trypsin Digestion]
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=Synechocystis sp. PCC6803 Protocols=
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[https://2012.igem.org/Team:WashU/Protocols/Transformation6803 Transformation Synechocystis 6803]
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[https://2012.igem.org/Team:WashU/Protocols/6803media Synechocystis 6803 media]
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=Carotenoid Measurement Protocols=
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[https://2012.igem.org/Team:WashU/Protocols/EthanolExtraction Carotenoid Extraction Using Ethanol]
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[https://2012.igem.org/Team:WashU/Protocols/ZeaxanthinExtraction Zeaxanthin Extraction Using Hexane]<br>

Latest revision as of 18:36, 17 August 2012


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