Team:Osaka/week3

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(Difference between revisions)
Line 10: Line 10:
#* 155(C):1-2D(upstream)+1-23L(downstream)+1-1A(vector)
#* 155(C):1-2D(upstream)+1-23L(downstream)+1-1A(vector)
# SDS-PAGE  
# SDS-PAGE  
 +
#* Gel electrophoresis
==August 16 (Thu)==
==August 16 (Thu)==

Revision as of 09:28, 17 August 2012

August 12 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation
    • 153(C):1-2M(upstream)+1-10H(downstream)+1-1A(vector)
    • 154(C):1-22A(upstream)+1-2M(downstream)+1-1A(vector)
    • 155(C):1-2D(upstream)+1-23L(downstream)+1-1A(vector)
  5. SDS-PAGE
    • Gel electrophoresis

August 16 (Thu)

  1. Transformaton of ligation products into DH5α E.coli:153~155
  2. Transformation of plasmid DNA into Rosetta E.coli:empty vector(A)

August 17 (Fri)

  1. Transfer to liquid culture

August 18 (Sat)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation

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