Team:Osaka/week2

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(Difference between revisions)
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==August 9 (Thu)==
==August 9 (Thu)==
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# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
# Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 3)
 +
# Transformation of plasmid DNA into Rosetta <i>E.coli</i>:147,148,150
 +
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
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|+Table3
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!ID!!Part Name!!Resistance!!Description
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|-
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|1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS
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|}
==August 10 (Fri)==
==August 10 (Fri)==
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+
# Transfer to liquid culture
==August 11 (Sat)==
==August 11 (Sat)==

Revision as of 09:27, 17 August 2012

August 5 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transfer to liquid culture
    • (Rosetta)143~146,149,152
    • (DH5α)150,Empty vector(A)

August 6 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformaton of Registry parts into DH5α E.coli(See Table 2)
  5. Preparation of glycerol stock of cell culture:143~146,149,152
Table2
IDPart NameResistanceDescription
1-2D<bbpart>BBa_J52008</bbpart>A,K Renilla Luciferase
1-10H<bbpart>BBa_I712019</bbpart>A,K Firefly Luciferase from Photinus pyralis
1-22A<bbpart>BBa_J23118</bbpart>A Constitutive promoter family member

August 7 (Tue)

  1. Preparation of LB agar plates (Cam)
  2. Transformation of 1-2D,1-10H

August 8 (Wed)

  1. Transfer to liquid culture:1-2D,1-10H,1-22A

August 9 (Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformaton of Registry parts into DH5α E.coli(See Table 3)
  5. Transformation of plasmid DNA into Rosetta E.coli:147,148,150
Table3
IDPart NameResistanceDescription
1-2M<bbpart>BBa_K118022</bbpart>ARBS

August 10 (Fri)

  1. Transfer to liquid culture

August 11 (Sat)

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