Team:UC Chile/Cyano/Labook/week5

From 2012.igem.org

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*Synechocystis PCC6803 culture was examined with acridine orange dye. It was axenic. Synechocystis was reinoculated in BG11 medium: 10 mL bacteria + 90 mL BG medium.
*Synechocystis PCC6803 culture was examined with acridine orange dye. It was axenic. Synechocystis was reinoculated in BG11 medium: 10 mL bacteria + 90 mL BG medium.
*E. coli was transformed with Gibson products.
*E. coli was transformed with Gibson products.
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Revision as of 20:04, 13 August 2012

Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012


April 2 - April 8


Tuesday


  • Electrophoresis gel was done with water instead of TAE.
  • PCR for parts to build C2.


Wednesday


  • Transformation of part K325005 (Lux operon).
  • Gibson assembly of C2.


Thursday


  • Synechocystis PCC6803 culture was examined with acridine orange dye. It was axenic. Synechocystis was reinoculated in BG11 medium: 10 mL bacteria + 90 mL BG medium.
  • E. coli was transformed with Gibson products.


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