Team:EPF-Lausanne/Notebook/12 July 2012

(Difference between revisions)

Latest revision as of 13:31, 13 August 2012

2 maxipreps using 200 ml of LB each, for RO + Spc. and LovTAP + Amp.

In the morning we realized that the bacteria had been overnight at 30.7ºC, so we left them 4 extra hours at 37ºC.

Centrifuge them for 20 min at 4000 rpm, thow away the supernatant and continue with the Maxiprep protocol.
In the last step, centrifuge the Falcon tubes at 4000 rpm, for one hour.
Bring the tubes to hood in the cell lab.
Remove the supernatant with the vacuum pump.
Add 5 ml of ethanol, shake gently, and remove with the vacuum pump. Be careful with the DNA pellet!
Leave the tubes open, at the back of the hood, with the label "DNA drying".

Forgot to insert protocol.

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+{{:Team:EPF-Lausanne/Template/LabPresence|Sander, Diego, Sowmya}}
 == Maxiprep ==
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 In the morning we realized that the bacteria had been overnight at 30.7ºC, so we left them 4 extra hours at 37ºC.
 * Centrifuge them for 20 min at 4000 rpm, thow away the supernatant and continue with the Maxiprep protocol.
+* In the last step, centrifuge the Falcon tubes at 4000 rpm, for one hour.
+* Bring the tubes to hood in the cell lab.
+* Remove the supernatant with the vacuum pump.
+* Add 5 ml of ethanol, shake gently, and remove with the vacuum pump. Be careful with the DNA pellet!
+* Leave the tubes open, at the back of the hood, with the label "DNA drying".
-{{:Team:EPF-Lausanne/Template/LabPresence|Sander, Diego, Sowmya}}
 <!-- Note: a list of all protocols can be found here: -->
@@ Line 18: / Line 24: @@
 {{:Team:EPF-Lausanne/Template/Protocol|None}}
-; Comments:
-Insert comments about what happened.
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