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05.07.12 transfection
Cell counting.
Supernatant sample stored:
- Well plate taken from Elisa room (CHB1374).
- Sampled 200 µl from each of the 5 cell cultures into 5 wells.
- Centrifuged at 1500 rpm, 3 min, in big centrifuge at Wiedner's lab (between E. coli and cell labs).
- In Elisa room, transfered 180 µl of supernatant from each sample into row A (columns 1 to 5 correspond to sample #) into new well plate, labeled Test(050712), kept in Elisa freezer (2nd shelf).
- Note: from now on, the plate stays out max 10 minutes! To avoid defreezing.
Protocol: None
Forgot to insert protocol.
- Comments
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