Team:Technion/8 August 2012
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==Lior== | ==Lior== | ||
Cip- me and Noa minipreped the Cip gene for the sequencing. we got 189 ng/ul <br> | Cip- me and Noa minipreped the Cip gene for the sequencing. we got 189 ng/ul <br> | ||
- | xyIE- the transformation worked, but apparantly we left them for too long in the 37 degrees so we got colonies with satelites (the bacteria threw the plasmid away. | + | xyIE- the transformation worked, but apparantly we left them for too long in the 37 degrees so we got colonies with satelites (the bacteria threw the plasmid away). So we took one colony that didnt have much satelites and made a starter for tomorrow. |
==Noa== | ==Noa== | ||
Revision as of 08:45, 9 August 2012
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Ilya
- Checked the sequencing results for BBa I0462. There is no way it is BBa I0462. A couple of days ago, I noticed that the experience info about this BioBrick states that it is actually a different BioBrick. Trying to clone this part was a waste of time. I will amplify the correct BBa I0462 from BBa F2620 using PCR and clone it downstream to R0062+RS+mCh.
- Glycerol stock of BBa F2620. Freezer position is 3C.
- Miniprep of BBa F2620. Concentrations are: 1F - 168.5 ng/ul, 2F - 169.5 ng/ul.
- The plate reader results seem to be weird. There is no change with concentration and the auto-fluorescence seems to be very high. Maybe the riboswitch doesn't function as expected.
Inbal
Asaf
I mini-preped the starter I have made yesterday of Puc19 12.1 containing the RiboSwitch.
The DNA concentration is 154.5 ng/µl.
Hila
Lior
Cip- me and Noa minipreped the Cip gene for the sequencing. we got 189 ng/ul
xyIE- the transformation worked, but apparantly we left them for too long in the 37 degrees so we got colonies with satelites (the bacteria threw the plasmid away). So we took one colony that didnt have much satelites and made a starter for tomorrow.