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Team:Cambridge/Lab book/Week 7
From 2012.igem.org
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'''[[Team:Cambridge/Protocols/PCRProtocol|PCR of Magnesium riboswitch vector fragment B and Magnesium promoter]]''' | '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of Magnesium riboswitch vector fragment B and Magnesium promoter]]''' | ||
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[[File:MgRS vector frag. B.jpg|right|250px|thumb|Riboswitch and promoter gel. Lanes 2+3: Fragment B without 8 codon substitution, lanes 4+5: Fragment B with 8 codon substitution, lanes 6+7: magnesium sensitive promoter, lane 8: +ve control]] | [[File:MgRS vector frag. B.jpg|right|250px|thumb|Riboswitch and promoter gel. Lanes 2+3: Fragment B without 8 codon substitution, lanes 4+5: Fragment B with 8 codon substitution, lanes 6+7: magnesium sensitive promoter, lane 8: +ve control]] | ||
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*Lane 5 accidentally loaded with a DNA ladder instead of loading dye. | *Lane 5 accidentally loaded with a DNA ladder instead of loading dye. | ||
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| + | *Expected fragment sizes: | ||
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| + | :*Lane 2-5: 3kbp | ||
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| + | :*Lane 6-7: 300bp | ||
*After electrophoresis, found vector products had, for the most part, worked. Promoter elements were not amplified - no band of the expected size was observed. | *After electrophoresis, found vector products had, for the most part, worked. Promoter elements were not amplified - no band of the expected size was observed. | ||
Revision as of 10:44, 7 August 2012
| Week: | 3 | 4 | 5 | 6 | 7 |
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Contents |
Monday
PCR of Magnesium riboswitch vector fragment B and Magnesium promoter
- Normal PCR settings used, annealing temperature 57 °C, elongation step 90s long.
- Lane 5 accidentally loaded with a DNA ladder instead of loading dye.
- Expected fragment sizes:
- Lane 2-5: 3kbp
- Lane 6-7: 300bp
- After electrophoresis, found vector products had, for the most part, worked. Promoter elements were not amplified - no band of the expected size was observed.
Tuesday
Wednesday
Thursday
Friday
