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Team:Evry/Freezer
From 2012.igem.org
(Difference between revisions)
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<td width="10%"> Name | <td width="10%"> Name | ||
| - | </td><td width=100> Sequence | + | </td><td width="100"> Sequence |
</td><td width="5%"> Length | </td><td width="5%"> Length | ||
</td><td width="5%"> Species of origin | </td><td width="5%"> Species of origin | ||
Revision as of 09:10, 6 August 2012
Freezer
Primers
| Number | Name | Sequence | Length | Tm | %GC | Comments | Date |
| P1 | Tir1-BB-FW | cgcttctagATGACGTACTTCCCGGAGG | 19 | 57 | 58 | 13th july | |
| P2 | Tir1-BB-Rv | ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC | 24 | 52 | 29 | 13th july | |
| P3 | SdMTir1-BB-Fw | AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG | 65 | 72 | 43 | 13th july | |
| P4 | SdMTir1-BB-Rv | TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG | 62 | 76 | 52 | 13th july | |
| P5 | Tir1 SdM v02-Fw | tgcTgtgacagaggagggac | 20 | 60 | 60 | 25th july | |
| P6 | Tir1 SdM v02-Fw | ctagAgcagcattagtcatttgg | 23 | 55 | 43 | 25th july | |
| P7 | pCS2+BB-Fw | cgctgcagGAACTATAGTGAGTCGTATTACG | 23 | 51 | 39 | 13th july | |
| P8 | pCS2+BB-Rv | tgcatGAATTCGAATCGATGGGATCC | 21 | 54 | 48 | 13th july | |
| P9 | GFP-AID-BB-Fw | gaattcgcggccgcttctagagATGGCCACAACCATGGTG | 40 | 74 | 58 | 20th july | |
| P10 | GFP-AID-BB-Rv | tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | 51 | 70 | 43 | 20th july | |
| P11 | p2 Hsp70-Fw | ACTGTCGACCCCGTTTAGCAGGAAATAGCC | 30 | 67 | 53 | Heat Shock | 20th july |
| P12 | p2 Hsp70-Rv | ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | 31 | 63 | 42 | Heat Shock | 20th july |
| P13 | p2 Flk-1-Fw | ACTGTCGACCTGGACTCTGGGCTAGGG | 27 | 68 | 63 | Vasculature | 20th july |
| P14 | p2 Flk-1-Rv | TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | 31 | 64 | 45 | Vasculature | 20th july |
| P15 | p2 ROSA-Fw | ACTGTCGACTAGATGAAGGAGAGCC | 25 | 61 | 52 | Ubiquitous | 20th july |
| P16 | p2 ROSA-Rv | GATAAGCTTGGATCCCCGCAAACGCAC | 27 | 66 | 56 | Ubiquitous | 20th july |
| P17 | p2 CarA-Fw | ACTGTCGACGTCCCATACCAGTACAATGCAAT | 32 | 66 | 47 | muscles | 20th july |
| P18 | p2 CarA-Rv | AGTAAGCTTGTGCTGTGATTGAATTGGCTG | 30 | 63 | 43 | muscles | 20th july |
| P19 | IaaH-BB-Fw | gaattcgcggccgcttctagATGCGCGAAATGATTACGC | 19 | 55 | 47 | 23th july | |
| P20 | IaaH-BB-Rv | ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | 25 | 52 | 28 | 23th july | |
| P21 | IaaM-BB-Fw | gaattcgcggccgcttctagatgtttggaccggatttccc | 20 | 56 | 50 | 23th july | |
| P22 | IaaM-BB-Rv | ctgcagcggccgctactagtattattagtcccccagcgc | 39 | 55/73 | 56/59 | 23th july | |
| P23 | AID-Fw | cgcttctagGGAGCTGGTGCAGGCGCT | 18 | 64 | 72 | 25th july | |
| P24 | Sp6 | ATTTAGGTGACACTATA | 17 | 41 | 27 | for BB detection when sequenced | NP |
| P25 | T3 | AATTAACCCTCACTAAAGGG | 20 | 50 | 40 | for BB detection when sequenced | NP |
| P26 | primer_sequencing_pCS2+_Fw | CAAGTGTAGCGGTCACGCTG | 20 | 59 | 60 | 13th july | |
| P27 | primer_sequencing_pCS2+_Rv | GCGTAATCATGGTCATAGCTG | 21 | 52 | 48 | 13th july | |
| P28 | GFP_AID_BB_Fw_v2 | TGCATgaattcgcggccgcttctagagATGGCCACAACCATGGTG | 45 | 75 | 56 | ||
| P29 | IaaH_BB_Fw_v2 | TGCATgaattcgcggccgcttctagATGCGCGAAATGATTACGC | 44 | 74 | 52 | ||
| P30 | IaaM_BB_Fw_v2 | TGCATgaattcgcggccgcttctagatgtttggaccggatttccc | 45 | 74 | 53 | ||
| P31 | IRES_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCATTCAagagaTCCCC | 50 | 53/74 | 50/52 | ||
| P32 | IRES_BB_R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgtggccatattatcatcg | 49 | 51/72 | 40/49 | ||
| P33 | BBa_G1005 | gtttcttcctgcagcggccgctactagta | 29 | 67 | 55 | ||
| P34 | IaaM_Kozak_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCATGCGCGAAATGATTACGC | 60 | 55/76 | 46/52 | ||
| P35 | IaaH_Kozak_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCatgtttggaccggatttcc | 60 | 54/76 | 47/52 |
Promoteurs
| Name | Sequence | Length | Species of origin | Expression domain | Restriction sites (EcoRI, XbaI, SpeI, PtsI) | Reference |
| p2 Xlurp | tctgtgtctccaaagatgccccagtagctccccatcttattttcactgcacatgctttgtactgctgtcacttactgagctcagggacccactcacaatatacagtacacatagaaatttcacagtataaggctgattagtaattaatacagataattactacatggaattacagaaaccagtgcaattagcatcagaatttaataatcagccctgtagcatcagcttatatgacaggccaacctcattttctgcttgataatttgcaacaactcctacgcttagcttctcaacagctgctcagagcccactgagcatgtgagagtcacagacactttccaagatggtgaccccctgtgacaggtttgaagtcctggatcattgctgctattgacaagctgaaactttaggctggtgcaataagttcagtatataaaacattgcaattctagccataataatttttagggtttagttctcctttaaggtggggccttgctaaagatgacttatttgcacatttataacatctgtcttaaaatagcatcttataactcatatacaaaaaaggggttatattgctacctacatgtcactcagcattaaaaagttccatgttccatgtttaagaggacatatttacaccgtttcaaacagtgaaatattctcatcttgctcgttggcaagaatgaggttaagtttgcagtgttgaataaaagataaatagtgattagctaaatttttcggcaggtttcattgtgaaaaagatgcccataggtgaaaaaaactgtgcgcaacaaaaaaattaattgtcgcccttcgccttcaatgcatttactgaccttttggcgaaacgagtcacattcgcccatcactaaagataaattaagtagtacacttccttcagtcttaaagtgcatcaacccaatgcaagatcatattgagctagaaggggaaggaatgccatatcatggataacacagggagataaatgtatctcctgtttgtagtagaggtcctatccatcagtaaaatatgtagacctctgcttcctagtggtaattgttgtacgcctcaacatccgtttctcaactagcaaagctttctcaaagcttattaccacctggagattaagcacctagatctagttattttccttgtgattgtgatatacagtatatgggacatcctagttggtaatttgcactacaggggaacaaaatggcatattcaggaatatatcagaaagaaaagcacatgcaaatagtgacaaacaatgcactgttaaaaaacaaaaaaagagaggcaggcctgttactctaggaaattcacctttaagagggagggagatataatttactaatatcagttaatttatttgaaagagaaaaagtagtaaaccccccactctctttaatacagtataaaaaaatattaaacagattgtgaacacaattaatgtggtgttaattcatatttgtgaattggtgtacaaacattcattgattaatcaaagtgcaagttcttcaatagtgcaatcaatgtccatgattttaagattaattaattatcttaaaaaaattattcaattagttcaatttttaaaagctgtaccctgcttgtaatctaattaactaaattcatctaataagtggaattaccgagtgtaattacagaaggtagcaattatattgaattaacaatatcaaattgaattcattaatttaaagtgctagtgcaaatataattaattagaaggtgaattaattcaatgaaagttaaaaatgtatgtccaggaccaacatctgaaaaaatgtaggaaaaggaaaataatagaggtggagaggtcgcaaaaacttctataagaggtttttctatgccctgggactccacacaatggagaagacaaacactggggactgtggtctcaaattaaccgtatctatctcattgtggagaggctaagctgtactataaaaccacagatccttcagtgtcattgaaagtaaggagaatttaaaggaagagaaaatcaaatgcggcacttgaaaggctaacagcgctggatattaattaaaagaaccaagccaattctaaaaagggtttggtagagaacagatttgttaaaatcaatttgaaagttaggagactccgccaatgcactgtaataagaaaaagtatatcagtttgcaggaaaacggagaaggcaaaactgggtggctaaaagaagtgagaaaaagaaggactagaaatgaaggggtggaaacctgagtggtgtagtaaaaagcaacaataaataaaaaaacacaacatagtgaaaatattttattgccatctgtttattaaaatctgtctattctgcacctgcaattctaaaatcattgtattctgaacagctgatattgcattgctctgttggaacttgaatggcggccctgtggctatttaagcttaattctataaacaatattagaaactcgaaaatgaacacacagaatttaccagtagaggattaaagtttattttatgtaaatgaatacaaaattcttactgtttttggttgctattggccgtttagatattttttaagcaaaacattagcaaagccctcaaagttaataattcatcggttaggccgtcctttattgatccatcaatcaggattcagttttgtgctgtcaaaaacaatgtgcgtgggaacaactttatgtggagcagggactcttcatgctctggtcagaaaatgtacaggaacttggaacagagaagaagtataaagaaaataatagcatgcatcacaatgtttctggtgtgccaatgaatagctgtgtgttacaaaatctctgtcttgtataaagacaacaccagctacagtaagttaaattctaaacacatatttgtccaatacaggggcccaattaatactgcttgtgcttttacggcacaatcaatctttaagaaaacccctcaagagtttcctaactcacgctggttttcatgctgaaaaagaaagctttgtaagttacccgacctgctgcaggactttatcccagggtgtattaataggagtttttacaataatggctcatggtaatactctgattaaactcaatagctcattgcaatactttgataaagaactgtataaaaatataacacaaagaactgttctgctgttgaacccgatgtcgtatgttgagcccaatatagagtaaaaagacagctttataacgtataattgtgtataacaaagctttgtatgatagtatacaaggcagccttataatccataatcctgtataatgaagcgttgtgcaagagaaatcatgtattatctaatatgcttatgatttctgaagcccttatcagactcttctttattataaataggggcacaatatgctattcagctaccagctagctaagataagatctctgtttcggatgtgcttgtgggaaacaagatctgttattaaccaaatcattatgagttattgactaagcctctcaatgagcttactgtttgaaagcatatataaactgggggtcaccatttggtcagttgagatctcacttgtggggttgacgcgtgccgccacaggaccttccgtgctccgtttgagggattcaatctgctgactgttccaggatctcccagacttggttgcagattgtttgctttgctcttttactcattcggtaatgtatatcttcttcattctatgtatgtaataaacctttgcttatactagctggtgtgtttgaattacttccgagagactcatagaaccacaattaaaacacagggtaacaaagtctgggcacccctgagctgacatagggcatctattgtctttgtccacagtcctgatatttttttatcaagcagtaactatgggcatgcaatttgcagtggggaaaacaagaatttggattggtgctagaactgtgtgctgctgaacaacaatgaaaactgccatcttaattctgctgaactgacacagctattttcttttttaaatatgtctattagttaaggggttaaactttaaaatgatgtagagagtaatatcccgagacaatttgctattggtttttattttttttactatttatggtatttgagctatttcgctttttattcagcagctctccagtttgcaattctggcgatctggttattaggatccaatgggtcagtgacccctgtctgaaagctgaaaagcctcctaagaagtaggcaagtcatttaaaaaacgatataaaaaaataaaataatgaagaccaagtgaaaagttgcttagaagtggccaatctatatcatacttaaagggatattgtcatgggaaaaacatttttttcaaaatgaatcagttaatagtgctgctccatcagaattctgcactgaaatccatttctcaaaagagcaaacagatttttttatattcaattttgaaatttgacatggggctagacatattgtcaatttcccagctgccccaagtcatgtgacttgtgctctgataaactttaatcactctttactgctgtactgcaagttagagtgatatcaccccctccctttttccccacagcagccaaacaaaagaacaaagggaaggtaatcagatagcagctccttaacacaagataacagctgcctggttagatataagaacaacactcaatagtaaaaacccatgtcccactgagacacattcagttacattgagaaggaaaaacagcagcctgccagaaagcatttctctctaaagtgcaggcacaagtcacatgacttggggcagctgggaaattgacaaaatgtctagccccatgtcagatttcaaaattgaatataaaaaaatctgtttgctcttttgagaaatggatttcagtgcaaaattctgctggagaagcactattaactatttgaaaaaaacatgttttctgatgacagtatccctttaaagttgacttcatggtaaaccacccctttgaaaacattacagactgtcagaaaagtatgtataaatggggaaaatatcaggaccacctaatttgagatttggtaggtatgtcactgtttgttacttttttcggttagtgaccttgcaacatttcctgtaacagcttatttcagtgtttacccttgttgatcaagggttacacaaattcccagaaatataacgtgcctttagtgattgtgcaatccatatagataaaaggggatcttgctggcagagttcacagaagagctgacttagaaacccact | 5273bp | Xenopus Laevis | Myeloid cells | / | Smith et al., 2002
|
Glycerol Stocks
| Number | Strain | Plasmid | Description | Date |
| P1 | E.Coli DH5 aplpha | pCS2+ | RFP ( BBA_J23100 ) | 13th july |
| P2 | E.Coli DH5 aplpha | pCS2+ | Auxine () | 13th july |
| P3 | E.Coli DH5 aplpha | pCS2+ | Tir1 GFP-Aid () | 19th july |
| P4 | E.Coli DH5 aplpha | pCS2+ | Tir1 GFP-Aid () | 19th july
|
