From 2012.igem.org
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| - | <h1>Weeks</h1>
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| - | <table>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w1">1</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w2">2</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w3">3</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w4">4</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w5">5</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w6">6</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w7">7</a></td>
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| - | <td>8</td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w9">9</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w10">10</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w11">11</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w12">12</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w13">13</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w14">14</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w15">15</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w16">16</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w17">17</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w18">18</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w19">19</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w20">20</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w21">21</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w22">22</a></td>
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| - | </tr>
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| | <h1>Week 8: 30th July - 5th August</h1> | | <h1>Week 8: 30th July - 5th August</h1> |
Revision as of 10:00, 3 August 2012
Weeks:
| June |
July |
August |
September |
October |
November |
Week 8: 30th July - 5th August
Monday, 30th July
Digestion - Ligation - Transformation:
Digestion of psc2+ and mRFP with EcoRI and PstI.
Ligation and Transformation on T10.
TirI BB
Electrophoresis of TirI last PCR: failure of this technique.
We buy a new primer to try another PCR technic.
Tuesday, 31st July
Inoculation:
Inoculation of pcs2+ (27.07) and repiquage of psc2+ with RFP (30.07)
Inoculation of psB1C3 with GFP-AID
TirI:
Electrophoresis of 27/07 results: Failure of second PCR
We restart the first step
Thursday, 2nd August
Sequencing
Send pCS2+ to sequencing.
Issue
We found that pCS2+ was badly made. We restart the construction of this plasmid from the beginning.
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