Team:Evry/Freezer
From 2012.igem.org
(Difference between revisions)
Line 50: | Line 50: | ||
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">P1 | <td style="background: #ccffcc;">P1 | ||
- | </td><td> | + | </td><td>Tir1-BB-FW |
</td><td>O3 Lox71-FtsA-F | </td><td>O3 Lox71-FtsA-F | ||
</td><td>O4 DEcoR1-FtsZ-R | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
</td><td>MG1655 | </td><td>MG1655 | ||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P3 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P4 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P5 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P6 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P7 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P8 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P9 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P10 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P11 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P12 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P13 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P14 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P15 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P2 | ||
+ | </td><td>Lox71-FtsA-FtsZ-1 | ||
+ | </td><td>O3 Lox71-FtsA-F | ||
+ | </td><td>O4 DEcoR1-FtsZ-R | ||
+ | </td><td>MG1655 | ||
+ | </td><td>MG1655 | ||
</table> | </table> |
Revision as of 14:52, 1 August 2012
Freezer
Primers
Number | Name | Length | Tm | %GC | Comments |
P1 | Tir1-BB-FW | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655
|
P3 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P4 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P5 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P6 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P7 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655
|
P8 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655
|
P9 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P10 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P11 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P12 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P13 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P14 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P15 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |
P2 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | MG1655 |