"
Team:Cambridge/Lab book/Week 6
From 2012.igem.org
(Difference between revisions)
(→Monday) |
(→Monday) |
||
| Line 14: | Line 14: | ||
Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive. | Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive. | ||
| - | PCR cycle x35 : *15s Denaturing at 95 C | + | PCR cycle x35: *15s Denaturing at 95 C |
| - | + | 45s Annealing at 60 C | |
| - | + | 300s Extension at 72 C | |
===Tuesday=== | ===Tuesday=== | ||
Revision as of 10:45, 30 July 2012
| Week: | 3 | 4 | 5 | 6 | 7 |
|---|
Contents |
Monday
Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.
PCR cycle x35: *15s Denaturing at 95 C
45s Annealing at 60 C
300s Extension at 72 C
Tuesday
Wednesday
Thursday
Friday
