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Team:Wageningen UR/Journal/week7
From 2012.igem.org
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*Prepare sample for PCR | *Prepare sample for PCR | ||
| - | *Run PCR | + | *Run PCR (98°C for 10 seconds, 55 °C for 30 seconds, 72 °C for 30 seconds, 35 cycles, pfusion enzyme) |
| - | *Prepare PCR-samples for gel | + | *Prepare PCR-samples for agarose gel |
| - | *Run gel | + | *Run agarose gel (1% w/v agarose) |
*Check gel | *Check gel | ||
Revision as of 08:07, 20 July 2012
week 7: 11 june - 15 may
Office work
Wiki page
Monday:
- update protocol and journal
Other
Tuesday:
- Preperations made for moving to a larger lab
[Meeting]
written by: Mark
Lab work
Bricking of CCMV and d26-CCMV second try
Monday:
- Prepare sample for PCR
- Run PCR (98°C for 10 seconds, 55 °C for 30 seconds, 72 °C for 30 seconds, 35 cycles, pfusion enzyme)
- Prepare PCR-samples for agarose gel
- Run agarose gel (1% w/v agarose)
- Check gel
Tecting CCMV protocol
Monday:
- Prepare samples for ultracentrifuge according to protocol
- Run ultracentrifuge
written by: Mark
