Team:UNITN-Trento

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<h2 style="width: 450px;"><span>We will be cleaning to save the World.</span> <span>One statue at a time.</span></h2>
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<h2 class="tk-chippewa-falls">Hi, we're the UniTN 2012 iGEM team!</h2>
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<p>Our project will be about statues and momuments. Here in Italy there are lots of them, magnificent and important. The problem is, with the modern society going on and everything, they get all ugly. Black ugly. We want to solve that. Ah, and protect them for the future, too.</p>
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<p>You know what the cool thing is? We'll engineer bacteria just to do that!</p>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Crust Away</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Terminator 5</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Attributions">Attributions</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Friends">Our Friends</a></li>
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      <h1><a href="https://2012.igem.org/Team:UNITN-Trento" style="font-family: 'Bree Serif';"><span>i</span>GEM Trento 2012</a></h1>
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<h2 class="tk-chippewa-falls">Project Codename: Statuary</h2><div class="medium_ribbon" ></div>
 
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<p>Statues and monuments all over the world are not enjoyed to the extent that they should be because they are covered in a disfiguring black crust. <br /> A practical solution would be if a cheap mechanism were available to continually remove the pollutant molecules that adhere to the surface of statues.  To build such a system, we are engineering E. coli to dissolve the disfiguring black crust, which consists mainly of CaSO4 (i.e. gypsum). <br /> More specifically, we are constructing an aerobic sulfur reducing pathway composed of a mutant E. coli serine acetyltransferase (CysE) that is insensitive to feedback inhibition and a Treponema denticola cysteine desulfhydrase.  CysE acetylates serine to give a precursor for cysteine synthesis, which also activates a sulfate assimilation pathway.  This pathway should then mobilize the SO4 of the black crust to make cysteine.  Finally, the cysteine desulfhydrase converts cysteine to H2S, pyruvate, and ammonia, thus completing the transformation of black crust sulfate to hydrogen sulfide gas.</p>
 
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<h2 class="tk-chippewa-falls">Project Codename: Terminator 5</h2><div class="medium_ribbon" ></div>
 
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<p>Surprisingly little standardization of T7 bacteriophage derived parts has occurred, despite the prevalence of T7 parts in recombinant technologies. <br /> Additionally, the compatibility between T7 and E. coli derived parts has not been explored.  For example, how efficient are T7 transcriptional terminators with E. coli RNA polymerase and is an E. coli transcriptional terminator effective with T7 RNA polymerase?  <br />To better understand the compatibility of the two systems and to begin to better characterize T7 biological parts, we are measuring the termination efficiencies of T7 and E. coli transcriptional terminators with E. coli and T7 RNA polymerases. <br /> To facilitate our measurements, we have developed a new biobrick compatible vector for ratiometric analyses by fluorescence spectroscopy and cell sorting.</p>
 
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<h2 style="text-align: center;">Saving the World, one statue at a time</h2>
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<h2 style="margin-bottom: -5px;">Our Project was selected among the Best 16 of the World Championship!</h2>
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<p>Our project had been awarded with a Gold Medal in Amsterdam and was among the "Sweet 16 High Scorers" in Boston! We're really proud and excited about it!</p>
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<h3>Project Codename: Crust Away</h3>
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<p>Statues and monuments all over the world are not enjoyed to the extent that they should be because they are covered in a disfiguring black crust, which consists mainly of CaSO<sub>4</sub>. <br />
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A practical solution would be if a cheap mechanism was available to continually remove the pollutant molecules that adhere to the surface of statues. To build such a system, we are engineering an aerobic sulfur reducing pathway in <i><i>E. coli</i></i> to dissolve the disfiguring black crust.</p>
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<span class="more"><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Read More <span class="arrow">&rarr;</span></a></span>
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<div class="two" style="margin-right: 0; width: 48%;">
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<h3>Project Codename: Terminator 5</h3>
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<p>Surprisingly little standardization of T7 phage derived parts has occurred, despite the prevalence of T7 parts in recombinant technologies.
 +
Also, the compatibility between T7 and <i>E. coli</i> derived parts has not been explored.
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To better understand the compatibility of the two systems and to begin to better characterize T7 biological parts, we are measuring the termination efficiencies of T7 and <i>E. coli</i> transcriptional terminators with <i>E. coli</i> and T7 RNA polymerases. </p>
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<span class="more"><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Read More <span class="arrow">&rarr;</span></a></span>
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<h3>MEET THE TEAM</h3>
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<div style="margin: 0 auto; width: 800px;"><a href="https://2012.igem.org/Team:UNITN-Trento/Team"><img src="http://www.science.unitn.it/~igem/img/team/Team.png" alt="" style="width: 800px; position: relative; left: -45px;" /></a></div>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team"><img src="http://www.science.unitn.it/~igem/img/team/Gugu.jpg" alt="" /></a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team"><img src="http://www.science.unitn.it/~igem/img/team/Giaco.jpg" alt="" /></a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team"><img src="http://www.science.unitn.it/~igem/img/team/Anna.jpg" alt="" /></a></li>
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<h3 style="text-align: center;">ACHIEVEMENTS</h3>
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<div style="margin: 0 auto; width: 800px;"><a href="https://2012.igem.org/Team:UNITN-Trento/Achievements"><img src="http://www.science.unitn.it/~igem/img/blackboard.png" alt="" style="width: 800px;" /></a></div>
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<li>&#10004;&nbsp;&nbsp; Submitted 21 Parts to the Registry</li>
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<li>&#10004;&nbsp;&nbsp; Fully characterized 15 Parts</li>
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<li>&#10004;&nbsp;&nbsp; Compiled an SRB Safety Handbook</li>
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<li>&#10004;&nbsp;&nbsp; Applied our aerobic sulfate reduction pathway on statues</li>
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<li>&#10004;&nbsp;&nbsp; Built a device to recreate black crust</li>
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Latest revision as of 13:18, 19 April 2013

iGEM Trento

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Saving the World, one statue at a time

Our Project was selected among the Best 16 of the World Championship!

Our project had been awarded with a Gold Medal in Amsterdam and was among the "Sweet 16 High Scorers" in Boston! We're really proud and excited about it!

Project Codename: Crust Away

Statues and monuments all over the world are not enjoyed to the extent that they should be because they are covered in a disfiguring black crust, which consists mainly of CaSO4.
A practical solution would be if a cheap mechanism was available to continually remove the pollutant molecules that adhere to the surface of statues. To build such a system, we are engineering an aerobic sulfur reducing pathway in E. coli to dissolve the disfiguring black crust.

Read More →

Project Codename: Terminator 5

Surprisingly little standardization of T7 phage derived parts has occurred, despite the prevalence of T7 parts in recombinant technologies. Also, the compatibility between T7 and E. coli derived parts has not been explored. To better understand the compatibility of the two systems and to begin to better characterize T7 biological parts, we are measuring the termination efficiencies of T7 and E. coli transcriptional terminators with E. coli and T7 RNA polymerases.

Read More →

MEET THE TEAM

ACHIEVEMENTS

Check out our Facebook page

Contact us by email

Retrieved from "http://2012.igem.org/Team:UNITN-Trento"