Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
(Difference between revisions)
(Created page with "== Strain Database == {| | Box ! Slot ! Name ! Description ! Source |- | iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab |- | iGEM 2012 Box 1 | 3 | BW25...") |
(→Strain Database) |
||
(66 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | + | {{Template:Austin_Texas/Stylesheet}} | |
- | {| | + | <html> |
- | + | <ul class="cssmenu"> | |
- | ! | + | <li class="home"><a href="/Team:Austin_Texas" title="home"><span class="displace">Home</span></a></li> |
+ | <li class="team"><a href="/Team:Austin_Texas/Team" title="team"><span class="displace">Team</span></a></li> | ||
+ | <li class="official_team_profile"><a href="https://igem.org/Team.cgi?year=2012&team_name=Austin_Texas" title="official_team_profile"><span class="displace">Official Team Profile</span></a></li> | ||
+ | <li class="project"><a href="/Team:Austin_Texas/Project" title="project"><span class="displace">Project</span></a></li> | ||
+ | <li class="parts_submitted"><a href="/Team:Austin_Texas/Parts" title="parts_submitted"><span class="displace">Parts Submitted</span></a></li> | ||
+ | <li class="modelling"><a href="/Team:Austin_Texas/Modeling" title="modeling"><span class="displace">Modeling</span></a></li> | ||
+ | <li class="notebook"><a href="/Team:Austin_Texas/Notebook" title="notebook"><span class="displace">Notebook</span></a></li> | ||
+ | <li class="safety"><a href="/Team:Austin_Texas/Safety" title="safety"><span class="displace">Safety</span></a></li> | ||
+ | <li class="attributions"><a href="/Team:Austin_Texas/Attributions" title="attributions"><span class="displace">Attributions</span></a></li> | ||
+ | </ul> | ||
+ | </html> | ||
+ | |||
+ | = Plasmid Database = | ||
+ | |||
+ | All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box. | ||
+ | |||
+ | {| cellpadding="5" border="1" cellspacing="0" | ||
+ | ! Box | ||
+ | ! Label | ||
! Name | ! Name | ||
+ | ! Part | ||
+ | ! Plasmid | ||
+ | ! Source | ||
+ | ! Sequence | ||
+ | ! Added by | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | | ||
+ | | cadA | ||
+ | | | ||
+ | | ASKA cadA plasmid | ||
+ | | Barrick Lab | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | | ||
+ | | ldcC | ||
+ | | | ||
+ | | ASKA ldcC plasmid | ||
+ | | Barrick Lab | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM001 | ||
+ | | LasR Promoter | ||
+ | | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | ||
+ | | pSB1A2 | ||
+ | | 2012 kit plate 1 (12A) | ||
+ | | Verified | ||
+ | | Razan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM002 | ||
+ | | LasR | ||
+ | | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | ||
+ | | pSB3K3 | ||
+ | | 2012 kit plate 1 (14J) | ||
+ | | Verified | ||
+ | | Razan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM003 | ||
+ | | Lac Promoter | ||
+ | | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | ||
+ | | pSB1A2 | ||
+ | | 2012 kit plate 1 (1D) | ||
+ | | | ||
+ | | Razan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM004 | ||
+ | | GFP | ||
+ | | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | ||
+ | | pSB3K3 | ||
+ | | 2012 kit plate 2 (15L) | ||
+ | | Verified | ||
+ | | Ben | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM005 | ||
+ | | RFP | ||
+ | | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | ||
+ | | pSB1AK3 | ||
+ | | 2012 kit plate 3 (12O) | ||
+ | | Verified | ||
+ | | Ben | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM006 | ||
+ | | LasI | ||
+ | | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | ||
+ | | pSB1A2 | ||
+ | | 2012 kit plate 2 (10N) | ||
+ | | Verified | ||
+ | | Ben | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM007 | ||
+ | | RBS + LacI | ||
+ | | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | ||
+ | | pSB1A2 | ||
+ | | 2012 kit plate 1 (7L) | ||
+ | | Verified | ||
+ | | Aurko | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM008 | ||
+ | | Double terminator (BBa_B0010 - BBa_B0012) | ||
+ | | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | ||
+ | | pSB1AK3 | ||
+ | | 2012 kit plate 1 (23L) | ||
+ | | Verified | ||
+ | | Aurko | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM009 | ||
+ | | J23100 promoter - sfGFP (Part:BBa_K515105) | ||
+ | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] | ||
+ | | pSB1C3 | ||
+ | | 2012 kit plate 5 (1K) | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM014 | ||
+ | | Plasmid pSB1A3 backbone with RFP Insert | ||
+ | | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | ||
+ | | pSB1A3 | ||
+ | | 2012 kit plate 1 (1G) | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM015 | ||
+ | | Plasmid pSB1C3 backbone with RFP Insert | ||
+ | | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | ||
+ | | pSB1C3 | ||
+ | | 2012 kit plate 1 (3A) | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM016 | ||
+ | | Plasmid pSB1K3 backbone with RFP Insert | ||
+ | | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | ||
+ | | pSB1K3 | ||
+ | | 2012 kit plate 1 (5A) | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM017 | ||
+ | | Plasmid pSB1T3 backbone with RFP Insert | ||
+ | | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | ||
+ | | pSB1T3 | ||
+ | | 2012 kit plate 1 (7A) | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM021 | ||
+ | | RFP | ||
+ | | [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] | ||
+ | | pSB1A2 | ||
+ | | 2012 kit plate 1 (13F) | ||
+ | | '''Incorrect''' | ||
+ | | Ben | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM022 | ||
+ | | | ||
+ | | | ||
+ | | pAK.TAQ | ||
+ | | Jared | ||
+ | | | ||
+ | | Mike | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM023 | ||
+ | | | ||
+ | | | ||
+ | | pET21.KOD | ||
+ | | Jared | ||
+ | | | ||
+ | | Mike | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM024 | ||
+ | | | ||
+ | | | ||
+ | | pQL269 | ||
+ | | Jared | ||
+ | | | ||
+ | | Mike | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM025 | ||
+ | | | ||
+ | | | ||
+ | | pACYC.T7.GFP | ||
+ | | Jared | ||
+ | | | ||
+ | | Mike | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM026 | ||
+ | | sfGFP | ||
+ | | | ||
+ | | pET21 | ||
+ | | | ||
+ | | | ||
+ | | Razan | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM027 | ||
+ | | Promoter-Theophylline riboswitch 1-Venus-Double terminator | ||
+ | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537009 BBa_K537009] | ||
+ | | pSB1C3 | ||
+ | | 2012 kit plate 5 (2M) | ||
+ | | '''Incorrect''' | ||
+ | | Ben | ||
+ | |- | ||
+ | | Plasmid Box 1 | ||
+ | | pIGM028 | ||
+ | | Theophylline Riboswitch 1-Venus | ||
+ | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537003 BBa_K537003] | ||
+ | | pSB1C3 | ||
+ | | 2012 kit plate 5 (7I) | ||
+ | | | ||
+ | | Peter/Ben | ||
+ | |} | ||
+ | |||
+ | = Strain Database = | ||
+ | |||
+ | All strains are in the back right rack of the –80°C freezer. | ||
+ | |||
+ | {| cellpadding="5" border="1" cellspacing="0" | ||
+ | ! Box | ||
+ | ! Slot | ||
+ | ! Label | ||
! Description | ! Description | ||
+ | ! Strain | ||
! Source | ! Source | ||
+ | ! Added by | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
Line 12: | Line 254: | ||
| AG1 | | AG1 | ||
| Host strain of ASKA collection | | Host strain of ASKA collection | ||
+ | | | ||
| Barrick Lab | | Barrick Lab | ||
+ | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
Line 18: | Line 262: | ||
| BW25113 | | BW25113 | ||
| Host strain of Keio collection | | Host strain of Keio collection | ||
+ | | | ||
| Barrick Lab | | Barrick Lab | ||
+ | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
Line 24: | Line 270: | ||
| cadA | | cadA | ||
| AG1 + ASKA collection cadA plasmid, CamR | | AG1 + ASKA collection cadA plasmid, CamR | ||
+ | | | ||
| Barrick Lab | | Barrick Lab | ||
+ | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
Line 30: | Line 278: | ||
| ldcC | | ldcC | ||
| AG1 + ASKA collection ldcC plasmid, CamR | | AG1 + ASKA collection ldcC plasmid, CamR | ||
+ | | | ||
| Barrick Lab | | Barrick Lab | ||
+ | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
Line 36: | Line 286: | ||
| tnaA | | tnaA | ||
| Keio collection knockout (version of odor-less E. coli), KanR | | Keio collection knockout (version of odor-less E. coli), KanR | ||
+ | | | ||
| Barrick Lab | | Barrick Lab | ||
+ | | | ||
|- | |- | ||
- | + | | iGEM 2012 Box 1 | |
+ | | | ||
+ | | IGM004 | ||
+ | | pSB3K3-I714891 (GFP - but not used in construct) | ||
+ | | TOP10 | ||
+ | | Barrick Lab | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM005 | ||
+ | | pSB1AK3-K081014 (RFP) | ||
+ | | TOP10 | ||
+ | | Barrick Lab | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM006 | ||
+ | | pSB1A2-K081009 (LasI) | ||
+ | | TOP10 | ||
+ | | Barrick Lab | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM009 | ||
+ | | crdASC (4864 bp) | ||
+ | | TOP10 | ||
+ | | Barrick Lab | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM010 | ||
+ | | crdASC (4943 bp) | ||
+ | | TOP10 | ||
+ | | Barrick Lab | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM011 | ||
+ | | crdASC (4864 bp) | ||
+ | | BL21(DE3) | ||
+ | | Barrick Lab | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM012 | ||
+ | | crdASC (4943 bp) | ||
+ | | BL21(DE3) | ||
+ | | Barrick Lab | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | 11 | ||
+ | | IGM013 | ||
+ | | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) | ||
+ | | epi300 | ||
+ | | Parts Kit | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM014 | ||
+ | | pSB1A3 with RFP Bba_J04450 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM015 | ||
+ | | pSB1C3 with RFP Bba_J04450 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM016 | ||
+ | | pSB1K3 with RFP Bba_J04450 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM017 | ||
+ | | pSB1T3 with RFP Bba_J04450 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM018 | ||
+ | | pSB1A2 with LasR Promoter Bba_R0079 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | |Razan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM019 | ||
+ | | pSB2K3 with LasR Bba_C0079 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | |Razan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM020 | ||
+ | | pSB1A2 with Lac Promoter Bba_R0010 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | |Razan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM021 | ||
+ | | J23151_GFP_pSB3K3: Bba_I20270 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | | Erik | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM022 | ||
+ | | J23101_GFP_pSB3K3: Bba_I20260 | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | | Erik | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM023 | ||
+ | | pet21.KOD | ||
+ | | TOP10 | ||
+ | | Jared | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM024 | ||
+ | | pQL269 | ||
+ | | TOP10 | ||
+ | | Jared | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM025 | ||
+ | | pACYC.T7.GFP | ||
+ | | TOP10 | ||
+ | | Jared | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM026 | ||
+ | | pSB1A2-J06504 (RFP--incorrect sequence) | ||
+ | | TOP10 | ||
+ | | Parts Kit | ||
+ | | Ben | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM027 | ||
+ | | pAK.TAQ | ||
+ | | TOP10 | ||
+ | | Jared | ||
+ | | | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM028 | ||
+ | | PAAV-miniCMV-mCherry | ||
+ | | Bl-21 AI | ||
+ | | Addgene | ||
+ | | Logan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM029 | ||
+ | | pET-SpA_ScTrp | ||
+ | | Bl-21 DE3 | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM030 | ||
+ | | pET-SpA_ScTrp_mCherry_5' | ||
+ | | Bl-21 AI | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM031 | ||
+ | | pET-SpA_ScTrp_mCherry_5' | ||
+ | | Bl-21 DE3 | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM032 | ||
+ | | pET-SpA_ScTrp_mCherry_3' | ||
+ | | Bl-21 DE3 | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM033 | ||
+ | | pET-SpA_ScTrp | ||
+ | | Bl-21 DE3 | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM034 | ||
+ | | pAAV-miniCMV-mCherry | ||
+ | | Bl-21 DE3 | ||
+ | | | ||
+ | | Logan | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM035 | ||
+ | | psb3k3-J2315.ORF1 + pra301-ndmApro | ||
+ | | TOP10 | ||
+ | | Barrick Lab | ||
+ | | Peter | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM036 | ||
+ | | J23001-NdmABCDgst9-pSB1C3 | ||
+ | | BW25113-guaB | ||
+ | | Erik | ||
+ | | Erik | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM037 | ||
+ | | psb3k3-J2315.ORF1 | ||
+ | | TOP10 | ||
+ | | Barrick Lab | ||
+ | | Peter | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM038 | ||
+ | | none | ||
+ | | BW25113-guaB | ||
+ | | Erik | ||
+ | | Erik | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM039 | ||
+ | | pSB1C3-K537011 | ||
+ | | TOP10 | ||
+ | | iGEM parts kit | ||
+ | | Peter | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM040 | ||
+ | | pSB1C3-K537003 | ||
+ | | TOP10 | ||
+ | | iGEM parts kit | ||
+ | | Peter | ||
+ | |- | ||
+ | | iGEM 2012 Box 1 | ||
+ | | | ||
+ | | IGM041 | ||
+ | | NdmABCD | ||
+ | | BW25113-guaB | ||
+ | | Erik | ||
+ | | Razan | ||
|} | |} |
Latest revision as of 20:22, 11 December 2012
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
Box | Label | Name | Part | Plasmid | Source | Sequence | Added by |
---|---|---|---|---|---|---|---|
Plasmid Box 1 | cadA | ASKA cadA plasmid | Barrick Lab | ||||
Plasmid Box 1 | ldcC | ASKA ldcC plasmid | Barrick Lab | ||||
Plasmid Box 1 | pIGM001 | LasR Promoter | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | pSB1A2 | 2012 kit plate 1 (12A) | Verified | Razan |
Plasmid Box 1 | pIGM002 | LasR | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | pSB3K3 | 2012 kit plate 1 (14J) | Verified | Razan |
Plasmid Box 1 | pIGM003 | Lac Promoter | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | pSB1A2 | 2012 kit plate 1 (1D) | Razan | |
Plasmid Box 1 | pIGM004 | GFP | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | pSB3K3 | 2012 kit plate 2 (15L) | Verified | Ben |
Plasmid Box 1 | pIGM005 | RFP | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | pSB1AK3 | 2012 kit plate 3 (12O) | Verified | Ben |
Plasmid Box 1 | pIGM006 | LasI | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | pSB1A2 | 2012 kit plate 2 (10N) | Verified | Ben |
Plasmid Box 1 | pIGM007 | RBS + LacI | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | pSB1A2 | 2012 kit plate 1 (7L) | Verified | Aurko |
Plasmid Box 1 | pIGM008 | Double terminator (BBa_B0010 - BBa_B0012) | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | pSB1AK3 | 2012 kit plate 1 (23L) | Verified | Aurko |
Plasmid Box 1 | pIGM009 | J23100 promoter - sfGFP (Part:BBa_K515105) | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] | pSB1C3 | 2012 kit plate 5 (1K) | ||
Plasmid Box 1 | pIGM014 | Plasmid pSB1A3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1A3 | 2012 kit plate 1 (1G) | Logan | |
Plasmid Box 1 | pIGM015 | Plasmid pSB1C3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1C3 | 2012 kit plate 1 (3A) | Logan | |
Plasmid Box 1 | pIGM016 | Plasmid pSB1K3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1K3 | 2012 kit plate 1 (5A) | Logan | |
Plasmid Box 1 | pIGM017 | Plasmid pSB1T3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1T3 | 2012 kit plate 1 (7A) | Logan | |
Plasmid Box 1 | pIGM021 | RFP | [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] | pSB1A2 | 2012 kit plate 1 (13F) | Incorrect | Ben |
Plasmid Box 1 | pIGM022 | pAK.TAQ | Jared | Mike | |||
Plasmid Box 1 | pIGM023 | pET21.KOD | Jared | Mike | |||
Plasmid Box 1 | pIGM024 | pQL269 | Jared | Mike | |||
Plasmid Box 1 | pIGM025 | pACYC.T7.GFP | Jared | Mike | |||
Plasmid Box 1 | pIGM026 | sfGFP | pET21 | Razan | |||
Plasmid Box 1 | pIGM027 | Promoter-Theophylline riboswitch 1-Venus-Double terminator | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537009 BBa_K537009] | pSB1C3 | 2012 kit plate 5 (2M) | Incorrect | Ben |
Plasmid Box 1 | pIGM028 | Theophylline Riboswitch 1-Venus | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537003 BBa_K537003] | pSB1C3 | 2012 kit plate 5 (7I) | Peter/Ben |
Strain Database
All strains are in the back right rack of the –80°C freezer.
Box | Slot | Label | Description | Strain | Source | Added by |
---|---|---|---|---|---|---|
iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab | ||
iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab | ||
iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab | ||
iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab | ||
iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab | ||
iGEM 2012 Box 1 | IGM004 | pSB3K3-I714891 (GFP - but not used in construct) | TOP10 | Barrick Lab | Ben | |
iGEM 2012 Box 1 | IGM005 | pSB1AK3-K081014 (RFP) | TOP10 | Barrick Lab | Ben | |
iGEM 2012 Box 1 | IGM006 | pSB1A2-K081009 (LasI) | TOP10 | Barrick Lab | Ben | |
iGEM 2012 Box 1 | IGM009 | crdASC (4864 bp) | TOP10 | Barrick Lab | Ben | |
iGEM 2012 Box 1 | IGM010 | crdASC (4943 bp) | TOP10 | Barrick Lab | Ben | |
iGEM 2012 Box 1 | IGM011 | crdASC (4864 bp) | BL21(DE3) | Barrick Lab | Ben | |
iGEM 2012 Box 1 | IGM012 | crdASC (4943 bp) | BL21(DE3) | Barrick Lab | Ben | |
iGEM 2012 Box 1 | 11 | IGM013 | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) | epi300 | Parts Kit | |
iGEM 2012 Box 1 | IGM014 | pSB1A3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
iGEM 2012 Box 1 | IGM015 | pSB1C3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
iGEM 2012 Box 1 | IGM016 | pSB1K3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
iGEM 2012 Box 1 | IGM017 | pSB1T3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
iGEM 2012 Box 1 | IGM018 | pSB1A2 with LasR Promoter Bba_R0079 | TOP10 | Parts Kit | Razan | |
iGEM 2012 Box 1 | IGM019 | pSB2K3 with LasR Bba_C0079 | TOP10 | Parts Kit | Razan | |
iGEM 2012 Box 1 | IGM020 | pSB1A2 with Lac Promoter Bba_R0010 | TOP10 | Parts Kit | Razan | |
iGEM 2012 Box 1 | IGM021 | J23151_GFP_pSB3K3: Bba_I20270 | TOP10 | Parts Kit | Erik | |
iGEM 2012 Box 1 | IGM022 | J23101_GFP_pSB3K3: Bba_I20260 | TOP10 | Parts Kit | Erik | |
iGEM 2012 Box 1 | IGM023 | pet21.KOD | TOP10 | Jared | ||
iGEM 2012 Box 1 | IGM024 | pQL269 | TOP10 | Jared | ||
iGEM 2012 Box 1 | IGM025 | pACYC.T7.GFP | TOP10 | Jared | ||
iGEM 2012 Box 1 | IGM026 | pSB1A2-J06504 (RFP--incorrect sequence) | TOP10 | Parts Kit | Ben | |
iGEM 2012 Box 1 | IGM027 | pAK.TAQ | TOP10 | Jared | ||
iGEM 2012 Box 1 | IGM028 | PAAV-miniCMV-mCherry | Bl-21 AI | Addgene | Logan | |
iGEM 2012 Box 1 | IGM029 | pET-SpA_ScTrp | Bl-21 DE3 | Logan | ||
iGEM 2012 Box 1 | IGM030 | pET-SpA_ScTrp_mCherry_5' | Bl-21 AI | Logan | ||
iGEM 2012 Box 1 | IGM031 | pET-SpA_ScTrp_mCherry_5' | Bl-21 DE3 | Logan | ||
iGEM 2012 Box 1 | IGM032 | pET-SpA_ScTrp_mCherry_3' | Bl-21 DE3 | Logan | ||
iGEM 2012 Box 1 | IGM033 | pET-SpA_ScTrp | Bl-21 DE3 | Logan | ||
iGEM 2012 Box 1 | IGM034 | pAAV-miniCMV-mCherry | Bl-21 DE3 | Logan | ||
iGEM 2012 Box 1 | IGM035 | psb3k3-J2315.ORF1 + pra301-ndmApro | TOP10 | Barrick Lab | Peter | |
iGEM 2012 Box 1 | IGM036 | J23001-NdmABCDgst9-pSB1C3 | BW25113-guaB | Erik | Erik | |
iGEM 2012 Box 1 | IGM037 | psb3k3-J2315.ORF1 | TOP10 | Barrick Lab | Peter | |
iGEM 2012 Box 1 | IGM038 | none | BW25113-guaB | Erik | Erik | |
iGEM 2012 Box 1 | IGM039 | pSB1C3-K537011 | TOP10 | iGEM parts kit | Peter | |
iGEM 2012 Box 1 | IGM040 | pSB1C3-K537003 | TOP10 | iGEM parts kit | Peter | |
iGEM 2012 Box 1 | IGM041 | NdmABCD | BW25113-guaB | Erik | Razan |