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| | <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li> | | <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li> |
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| | <font face="Arial, Helvetica"><br> | | <font face="Arial, Helvetica"><br> |
| | </font><h3 class="STYLE2"><font face="Arial, Helvetica">1. Fluorescence microscope results</font></h3> | | </font><h3 class="STYLE2"><font face="Arial, Helvetica">1. Fluorescence microscope results</font></h3> |
| - | Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures (Figure 1 and Figure 2) show that both GFP and RFP are expressed. | + | <p>Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.<p> |
| - | <p class="STYLE2"><font face="Arial, Helvetica"><img src=" https://static.igem.org/mediawiki/2012/ d/ d2/ 71.png" class="img_fl img_border" //font> </p> | + | <img src=" https://static.igem.org/mediawiki/2012/e/ec/81.png" class="img_fl img_border" > |
| - | | + | <p><span class="STYLE3">(Figure 1 Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. ) </span></p> |
| - | <p><span class="STYLE3">(Figure 1: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the RFP. )</span></p> | + | |
| - | <p class="STYLE2"><font face="Arial, Helvetica"><img src=" https://static.igem.org/mediawiki/2012/e/ec/81.png" class="img_fl img_border" //font></p>
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| - | <p><span class="STYLE3">(Figure 2: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. ) </span></p>
| + | <h3 class="STYLE2"><font face="Arial, Helvetica">2.Flow cytometry results </font></h3> |
| - | <p> </p>
| + | <img src=" https://static.igem.org/mediawiki/2012/6/60/Lab_results-f1.jpg" class="img_fl img_border" > |
| - | <h3 class="STYLE2"><font face="Arial, Helvetica">2. Fit curves relating to dscores and terminator efficiencies. </font></h3>
| + | <p><span class="STYLE3">Figure2-a</span></p> |
| - | <p class="STYLE4"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/6/ 6d/ QQ%E6%88%AA%E5%9B%BE20120927115928. png" class="img_fl img_border" //font />< /span></p> | + | <img src=" https://static.igem.org/mediawiki/2012/6/60/Lab_results-f2.jpg" class="img_fl img_border" > |
| - | <p class="STYLE3">(figure 6: Summary of results)</p> | + | <p><span class="STYLE3">Figure2-b</span></p> |
| - | <p class="STYLE2"><img src="https://static.igem.org/mediawiki/2012/ e/ ec/ 91. png" class="img_fl img_border" //font />< /p> | + | <p>Flow cytometry results of GFP expression in experiment group Figure2-a and control group Figure2-b.The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58</p> |
| - | <p class="STYLE3">(figure 3 : According the data on biofab ( <a href="http://io.biofab.org/services/studio/dac/">http://io.biofab.org/services/studio/dac/</a> ) and our software predicted dscores, create fit curves. Linear fit curve is founded to have the highest accuracy among all the fit curves. )</p> | + | <h3 class="STYLE2"><font face="Arial, Helvetica">3.Agreement with theoretical prediction</font></h3> |
| - | <p class="STYLE3"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/d/d6/92.png" class="img_fl img_border" //font /></span></p> | + | <p class="STYLE3"><span class="STYLE2"><img src="https://static.igem.org/mediawiki/2012/d/d5/Labresult.jpg" class="img_fl img_border" //font /></span></p> |
| - | <p class="STYLE3">(figure 4 : According to our experiment measured terminator efficiencies and our software predicted dscores, we create fit curve to relate all the data . To correspond to the figure 3, we choose linear fit curve to link all the data. There are 5 valuable points of terminator efficiencies. The sequences are listed below. In the experiment, we transform 9 terminators to plasmid mutant-psb1a3-GF and only get 5 reliably terminators’ data. The points appeared on the figure are terminators whose number is 1,4,6,7,8 )</p> | + | <p class="STYLE3">(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)</p> |
| | <p class="STYLE4"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/2/2b/94.png" class="img_fl img_border" //font /></span></p> | | <p class="STYLE4"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/2/2b/94.png" class="img_fl img_border" //font /></span></p> |
| - | <p class="STYLE3">(figure 5 : Here listed the sequences of terminator whose efficiencies are measured in the lab.)</p> | + | <p class="STYLE3">(Figure 4 Here listed the sequences of terminator whose efficiencies are measured in the lab.)</p> |
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