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| {{:Team:Edinburgh/header}} | | {{:Team:Edinburgh/header}} |
| + | {{Team:Edinburgh/css/navigation-structure.css}} |
| + | {{Team:Edinburgh/css/navigation-style.css}} |
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| <html> | | <html> |
| <style type="text/css"> | | <style type="text/css"> |
- | /* wiki edit */ | + | /* header edit */ |
- | #content{
| + | |
- | /*background-image: url('https://static.igem.org/mediawiki/2012/5/5b/Edi-project-bg-line-01.JPG');*/
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- | /*https://static.igem.org/mediawiki/2012/5/5b/Edi-project-bg-line-01.JPG*/
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- | /*https://static.igem.org/mediawiki/2012/b/b9/Edi-project-bg-2.JPG*/
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- | /*https://static.igem.org/mediawiki/2012/c/c6/Edi-project-page-bg.jpg*/
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- | /*background-repeat: repeat-x;*/
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- | }
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- | #project-middle{
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- | background-image: url('https://static.igem.org/mediawiki/2012/d/de/Edi-project-bg-5.png');
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- | background-repeat: no-repeat;
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- | }
| + | |
- | | + | |
- | /*style - header edit*/
| + | |
| #project-page a{ | | #project-page a{ |
| + | display:block; |
| background-color: #f2f2f2; | | background-color: #f2f2f2; |
| border-top-right-radius: 10px; | | border-top-right-radius: 10px; |
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| } | | } |
| #project-page a, | | #project-page a, |
- | #project-page a:visited, | + | #project-page a:visited{ |
- | #project-page a:hover{
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| color: #000; | | color: #000; |
| } | | } |
| #project-page a:hover{ | | #project-page a:hover{ |
| cursor: default; | | cursor: default; |
| + | color: #000; |
| } | | } |
- | /*structure*/ | + | /* actual page */ |
- | #project-middle{ | + | #page-content{ |
- | width:1085px; | + | background-color:#fff; |
- | margin-right:auto; | + | /*background-image: url('https://static.igem.org/mediawiki/2012/d/de/Edi-project-bg-5.png'); |
- | margin-left:auto; | + | background-repeat: no-repeat;*/ |
- | border:none; | + | border:1px solid #ccc; |
- | padding-bottom:30px;
| + | |
| } | | } |
- | /* style */
| + | #project-abstract-video{ |
- | #project-middle p{ | + | |
- | padding:20px;
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- | font: normal 12px/20px verdana, tahoma, sans-serif;
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- | text-align:justify;
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- | background-color: rgba(242,242,242,0.8);
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- | border-radius:20px;
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- | border: 1px solid #3f3f3f;
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- | }
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- | #introduction{
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- | width:1085px;
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| margin-top:20px; | | margin-top:20px; |
- | }
| + | margin-left:120px; |
- | #bioel-interface{
| + | border:1px solid #a8a8a8; |
- | width:600px;
| + | padding:4px; |
- | margin-top:30px; | + | border-radius:4px; |
- | margin-left:280px; | + | box-shadow:0px 0px 5px #a8a8a8; |
- | }
| + | background:#f2f2f2; |
- | #selectable-markers{
| + | width:560px; |
- | width:985px; | + | |
- | margin-top:40px; | + | |
- | margin-left:100px; | + | |
- | }
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- | #lemon-bacteria{
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- | width:580px; | + | |
- | margin-top:40px; | + | |
| } | | } |
| </style> | | </style> |
| + | </html> |
| | | |
- | <div id="project-middle">
| + | {{:Team:Edinburgh/Project/navigation}} |
- | <!--img src="https://fbcdn-sphotos-a.akamaihd.net/hphotos-ak-ash3/577406_10150875741121899_1000746002_n.jpg"-->
| + | |
- | <div class="project-description" id="introduction">
| + | |
- | <p>
| + | |
- | In the spirit of iGEM, our project’s aim is to design new biological systems that will make synthetic biology more accessible and friendly. Our team plans to achieve this by constructing a bio-electric interface, designing new selectable and counterselectable markers and characterising Citrobacter freundii as a new chassis. In addition, we want to research the subject of Open Source and DIY biology in relation to the laboratory part of our project as well as its future potential.
| + | |
- | </p>
| + | |
- | </div><!-- /introduction -->
| + | |
- | <div class="project-description" id="bioel-interface">
| + | |
- | <p>
| + | |
- | <b>Bioelectric interface:</b> As part of our project we will attempt to create a bioelectric interface - a way to connect biological and electronic systems in a standardised, inducible and quantifiable way.
| + | |
- | To achieve our goal we will use the MtrCAB genes (cytochromes, proteins that mediate electron transport) from Shewanella oneidensis. We will transform E. coli with these genes along with a ccm gene cluster (cytochrome c maturation proteins) and couple it to an inducible promoter such as Ars or Lac promoter induced by arsenate or lactose/IPTG.
| + | |
- | As a result, we should be able to obtain a system that would allow us to measure the rate of electron export in response to an input of arsenate or IPTG. We will model the process using Kappa and MATLAB. Possible methods to measure electron export include construction of a microbial fuel cell or use of the ferrozine assay to measure rate of reduction of iron (III) ions to iron (II).
| + | |
- | </p>
| + | |
- | </div><!-- /bioel-interface -->
| + | |
- | <div class="project-description" id="selectable-markers">
| + | |
- | <p>
| + | |
- | We are also looking at two ways of making genetically modified bacteria safer to release into the environment:<br />
| + | |
| | | |
- | <b>Selectable and counter-selectable markers:</b> Alternative to antibiotic resistance: We are investigating other ways to distinguish between the cells which have taken up the gene in question and those which have not in order to eliminate the need of antibiotic resistance selection. This would reduce the spreading of antibiotic resistance genes if an engineered bacterium were to be released into the environment. | + | <html> |
- | </p>
| + | <div id="page-content"> |
- | </div><!-- /selectable-markers -->
| + | <div class="text"> |
- | <div class="project-description" id="lemon-bacteria">
| + | <p class="normal-text"> |
- | <p>
| + | In the spirit of iGEM, our project’s aim is to design new biological systems that will make synthetic biology more accessible and friendly. Our team plans to achieve this by constructing a bio-electric interface, designing new selectable and counterselectable markers and characterising <i>Citrobacter freundii</i> to start a dialogue on what a synthetic-biology specific chassis should look like. <span class="intense-emphasis">For more detailed information on each of these sub-projects, refer to the links in the navigation menu on the left.</span> |
- | <b>Chassis characterization:</b> Citrobacter freundii. We intend to characterize this 'friendly lemon bacterium' (a member of the gamma-proteobacteria, like Escherichia coli) in order to assess whether it would be a good chassis for cloning and gene expression. The emphasis will be on testing whether BioBricks designed for E. coli are compatible with Citrobacter. We also aim to characterize other criteria which would have to be known in order for researchers to start using it as a novel chassis. In addition to characterizing its growth requirements and BioBrick compatibility, we hope to sequence its genome to gain more insight into its metabolic pathways and novel genes. Finally, we want to assess whether public opinion would favour less known but safer Citrobacter freundii over Escherichia coli, which may have a bad reputation due to its association with disease and sewage and ability to become pathogenic if exposed to wild type strains.
| + | <br /><br /> |
- | </p>
| + | However, if you are short for time, you may want to have a look at <span class="plainlinks"><a href="http://dl.dropbox.com/u/108285418/EdiGEM%20-%20iGEM%20Edinburgh%202012.pdf"><b>EdiGEM's Concise Project Description</b></a></span>. |
- | </div><!-- lemon-bacteria --> | + | </p> |
- | | + | <p class="h1"> |
- | | + | Project Abstract |
- | </div><!-- /project-middle --> | + | </h1> |
| + | <div id="project-abstract-video"> |
| + | <iframe class="project-abstract" width="560" height="315" src="http://www.youtube.com/embed/wnd77MsyMi4" frameborder="0" allowfullscreen></iframe> |
| + | </div><!-- /project-abstract-video --> |
| + | <p class="h2"> |
| + | <a href="https://2012.igem.org/Team:Edinburgh/Project/Bioelectric-Interface">Bioelectric interface</a> |
| + | </p> |
| + | <p class="normal-text"> |
| + | As part of our project we will attempt to create a bioelectric interface - a way to connect biological and electronic systems in a standardised, inducible and quantifiable way. |
| + | <br /><br /> |
| + | To achieve our goal we will use the MtrCAB proteins (cytochromes, proteins that mediate electron transport) from <i>Shewanella oneidensis</i>. We will transform <i>E. coli</i> with these genes along with a <i>ccm</i> gene cluster (cytochrome c maturation proteins) and couple it to an inducible promoter such as the Ars or Lac promoters which are induced by arsenate or lactose/IPTG. |
| + | <br /><br /> |
| + | As a result, we should be able to obtain a system that would allow us to measure the rate of electron export in response to an input of arsenate or IPTG. Possible methods to measure electron export include measuring the transfer of electrons to an electrode with a volt meter by comparing it to a reference electrode; construction of a microbial fuel cell or the use of the ferrozine assay to measure the rate of reduction of iron (III) ions to iron (II). |
| + | <br /><br /> |
| + | We are also looking at two ways of making genetically modified bacteria safer to release into the environment: |
| + | </p> |
| + | <p class="h2"> |
| + | <a href="https://2012.igem.org/Team:Edinburgh/Project/Non-antibiotic-Markers">Alternative Selectable and counter-selectable markers</a> |
| + | </p> |
| + | <p class="normal-text"> |
| + | Alternative to antibiotic resistance: We are investigating other ways to distinguish between the cells which have taken up the plasmid in question and those which have not in order to eliminate the need for antibiotic resistance selection. This would reduce the spreading of antibiotic resistance genes if an engineered bacterium were to be released into the environment either deliberately or by accident. |
| + | </p> |
| + | <p class="h2"> |
| + | <a href="https://2012.igem.org/Team:Edinburgh/Project/Citrobacter-Freundii">Chassis characterization: <i>Citrobacter freundii</i></a> |
| + | </p> |
| + | <p class="normal-text"> |
| + | We intend to characterize this 'friendly lemon bacterium' (a member of the gamma-proteobacteria, like <i>Escherichia coli</i>) in order to assess whether it would be a good chassis for cloning and gene expression within synthetic biology and beyond. We want to see what it can offer to this field to assess whether there are some new things it can do, or can do better than <i> E. coli </i>, the legacy chassis. We want to start a dialogue about what a synthetic biology-specific chassis should look like, what it should be able to do and what should be known about it before it could be considered a good alternative for the currently existing chassis. |
| + | <br /><br /> |
| + | We also aim to characterize various criteria which would have to be known in order for researchers to start using it as a novel chassis. In addition to characterizing its growth requirements and BioBrick compatibility, we hope to sequence its genome to gain more insight into its metabolic pathways and novel genes. |
| + | <br /><br /> |
| + | Finally, we want to assess whether public opinion would favour the less known but safer <i>Citrobacter freundii</i> over <i>Escherichia coli</i>, which may have a bad reputation due to its association with disease, sewage and ability to become pathogenic if exposed to wild type strains. |
| + | </p> |
| + | </div><!-- /text --> |
| + | </div><!-- /page-content --> |
| + | </div><!-- /page-middle --> |
| </html> | | </html> |