Team:NCTU Formosa/Project

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      <h1><a href="#sub1">Introduction</a></h1>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project">Introduction</a></h1>
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      <p>Introduction</p>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project"><p>Introduction</p></a>
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      <h1><a href="#sub2-1">Enzyme for Isobutanol</a></h1>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-1">Enzyme for Isobutanol</a></h1>
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      <p>Enzyme for Isobutanol</p>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-1"><p>Enzyme for Isobutanol</p></a>
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      <h1><a href="#sub2-2">Temperature Control System</a></h1>
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      <p>Temperature Control System</p>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-2"><p>Temperature Control System</p></a>
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      <h1><a href="#sub2-3">Zinc Finger</a></h1>
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      <p>Zinc Finger</p>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-3"><p>Zinc Finger</p></a>
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      <h1><a href="#sub2-4">Instrument</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-4"><p>Instrument</p></a>
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      <h1><a href="#sub3">Conclusion</a></h1>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub3">Conclusion</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub3"><p>Conclusion</p></a>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub4">Optimization</a></h1>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub5">Future Works</a></h1>
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<h1 id="project-s1-title" class="project-s-title"><a name="sub1"> </a>　<span>Introduction to the project</span></h1>
<h1 id="project-s1-title" class="project-s-title"><a name="sub1"> </a>　<span>Introduction to the project</span></h1>
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<p>Nowadays, environmental pollution and energy depletion have become crucial problems. We need to find alternative energy to replace the running out fossil fuel. Due to the pollution issues, this alternative energy should be environmental friendly. Up until now, ethanol is the most common biomass fuel because the final product is harmless water. However, ethanol will corrode metallic  surface of the engines lead to higher cost than fossil fuel usage. Unlike ethanol, isobutanol do not corrode metal and contain higher ratio of the heat of combustion than ethanol. Besides, as well as ethanol, isobutanol doesn’t produce pollutants such as sulfur dioxide, nitric oxide and nitric dioxide. Isobutanol has widely utilized in many applications as a organic solvent, and antifreeze. As what we want, to get clean energy, we chose isobutanol to be our project. We believe that isobutanol is a potential eco fuel in the future. However, currently isobutanol production wasn't very promising. According to the previous studies, the low yield of isobutanol was caused by the toxicity of isobutanol which would kill the host <i>E.coli</i> . In this study, we introduced two innovative and brilliant solutions to solve this serious problem. Now, let’s take a deeper look in our new ideas!</p></div>
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<h2 id="project-s2-1-title" class="project-s-title"><a name="sub2-1"> </a>　<span>Enzyme for isobutanol</span></h2>
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<p>The low temperature release system is a way to let e.coli produce isobutanol efficiently . Because isobutanol and isobutyaldehyde are toxic to the e.coli , the system avoid e.coli facing them at the beginning . The following picture is our system.</p>
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<p>At the beginning , we will let E.coli stay in 37°C environment. After having enough 2-Ketoisovalerate , we will move E.coli into 30°C environment for producing the final product , isobutanol. It can make us get isobutanol successfully and efficiently.</p>
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<p>This is our biobrick. The most important part of our biobrick is 37°C ribosome binding site gene. We separate our biobrick into two parts . The first part is which has 37℃ ribosome binding site gene and the second part is under the first one.</p>
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<p>And now we're introducing how our system works.</p>
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<p>When being in 37°C environment, the first part will be translated and produce tetR protein to inhibit Ptet promoter. The second part will not be translated. Then we can produce intermediate , 2-Ketoisovalerate.</p>
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<p>After getting enough 2-Ketoisovalerate , E.coli will stay in 30°C environment. The ribosome will not bind the 37°C ribosome binding site and tetR genes will not be translated. Then the second part will be translated successfully. At the end , we can get the isobutanol.</p>
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<h2 id="project-s2-s-title" class="project-s-title">　<span>Result</span></h2>
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<p>The report shows that we use fluorescene protein to mark the second part of our biobrick . We can see that fluorescene protein staying in 30°C environment will have higher expression than staying in 37°C environment . According to it , we can see that our system do truly work!</p>
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<h2 id="project-s2-3-title" class="project-s-title"><a name="sub2-3"> </a>　<span>Zinc finger</span></h2>
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<h2 id="project-s2-s-title" class="project-s-title">　<span>Result</span></h2>
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<p>(developing)</p>
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<h2 id="project-s2-4-title" class="project-s-title"><a name="sub2-4"> </a>　<span>Instrument</span></h2>
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<p>(developing)</p></div>
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<h1 id="project-s3-title" class="project-s-title"><a name="sub3"> </a>　<span>Conclusion</span></h1>
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<p>(developing)</p></div>
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     <p>Copyright © 2012 NCTU Formosa</p>
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<p>Background image belongs to <a href="http://fatezoom.deviantart.com/" target="_blank">Yuri Kormin</a>.</p>
<p>Background image belongs to <a href="http://fatezoom.deviantart.com/" target="_blank">Yuri Kormin</a>.</p>

Latest revision as of 15:15, 26 October 2012

Team:NCTU Formosa - 2012.igem.org

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　Introduction to the project

Nowadays, environmental pollution and energy depletion have become crucial problems. We need to find alternative energy to replace the running out fossil fuel. Due to the pollution issues, this alternative energy should be environmental friendly. Up until now, ethanol is the most common biomass fuel because the final product is harmless water. However, ethanol will corrode metallic surface of the engines lead to higher cost than fossil fuel usage. Unlike ethanol, isobutanol do not corrode metal and contain higher ratio of the heat of combustion than ethanol. Besides, as well as ethanol, isobutanol doesn’t produce pollutants such as sulfur dioxide, nitric oxide and nitric dioxide. Isobutanol has widely utilized in many applications as a organic solvent, and antifreeze. As what we want, to get clean energy, we chose isobutanol to be our project. We believe that isobutanol is a potential eco fuel in the future. However, currently isobutanol production wasn't very promising. According to the previous studies, the low yield of isobutanol was caused by the toxicity of isobutanol which would kill the host E.coli . In this study, we introduced two innovative and brilliant solutions to solve this serious problem. Now, let’s take a deeper look in our new ideas!

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