Team:Wageningen UR/Protocol

From 2012.igem.org

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= Protocol =
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= Methods =
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The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.
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<ul>
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<li>[[Team:Wageningen_UR/MethodsProduction|Production]]</li>
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<li>[[Team:Wageningen_UR/MethodsPurification|Purification]]</li>
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</ul>
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= Protocols =
== Medium & Buffer recipes ==
== Medium & Buffer recipes ==
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*[[Team:Wageningen_UR/Protocol/LBmedium|LB medium]]
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<ul>
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*[[Team:Wageningen_UR/Protocol/SOBmedium|SOB medium]]
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<li>[[Team:Wageningen_UR/Protocol/LBmedium|LB medium]]</li>
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*[[Team:Wageningen_UR/Protocol/SOCmedium|SOC medium]]
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<li>[[Team:Wageningen_UR/Protocol/SOBmedium|SOB medium]]</li>
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*[[Team:Wageningen_UR/Protocol/Disassemblybuffer|Disassembly buffer]]
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<li>[[Team:Wageningen_UR/Protocol/SOCmedium|SOC medium]]</li>
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*[[Team:Wageningen_UR/Protocol/Dialysisbuffer10x|Dialysis buffer 10x]]
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<li>[[Team:Wageningen_UR/Protocol/Disassemblybuffer|Disassembly buffer]]</li>
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*[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]
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<li>[[Team:Wageningen_UR/Protocol/Dialysisbuffer10x|Dialysis buffer 10x]]</li>
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*[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]
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<li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li>
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*[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]
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<li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li>
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*[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]
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<li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li>
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<li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li>
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<li>[[Team:Wageningen_UR/Protocol/FormationBufferPolero|Formation Buffer Polero]]</li>
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<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
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<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>
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</ul>
== CCMV Coat Protein VLP formation ==
== CCMV Coat Protein VLP formation ==
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*[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]
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<ul>
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*[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]
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<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li>
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*[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifing the VLPs]]
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<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifying the VLPs]]</li>
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</ul>
== Hepatitis B Coat Protein VLP formation ==
== Hepatitis B Coat Protein VLP formation ==
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== PoLeRo Coat Protein VLP formation ==
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<ul>
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<li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li>
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<li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupHepB|Purifying the VLPs]]</li>
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</ul>
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== Polerovirus Coat Protein VLP formation ==
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<ul>
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<li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li>
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<li>[[Team:Wageningen_UR/Protocol/StartupPolero|Growing culture]]</li>
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<li>[[Team:Wageningen_UR/Protocol/DialysisPolero|Dialysis of the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupPolero|Purifying the VLPs]]</li>
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</ul>
== General Protocol ==
== General Protocol ==
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*[[Team:Wageningen_UR/Protocol/ElectrocompetentCells|Preparation of electrocompetent cells of ''E. coli'']]
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<ul>
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*[[Team:Wageningen_UR/Protocol/SDSPolyacrylamide|SDS-Polyacrylamide and native gel electrophoresis]]
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<li>[[Team:Wageningen_UR/Protocol/ElectrocompetentCells|Preparation of electrocompetent cells of ''E. coli'']]</li>
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*[[Team:Wageningen_UR/Protocol/DynamicLightScatteringMachine|Dynamic Light Scattering user manual]]
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<li>[[Team:Wageningen_UR/Protocol/SDSPolyacrylamide|SDS-Polyacrylamide and native gel electrophoresis]]</li>
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*[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]
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<li>[[Team:Wageningen_UR/Protocol/DynamicLightScatteringMachine|Dynamic Light Scattering user manual]]</li>
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*[[RNA isolation from leaf material]]
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<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li>
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<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li>
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<li>[[Team:Wageningen_UR/Protocol/Mutagenesis|Mutagenesis]]</li>
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</ul>

Latest revision as of 10:44, 26 October 2012

Contents

Methods

The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.

Protocols

Medium & Buffer recipes

CCMV Coat Protein VLP formation

Hepatitis B Coat Protein VLP formation

Polerovirus Coat Protein VLP formation

General Protocol

Retrieved from "http://2012.igem.org/Team:Wageningen_UR/Protocol"