Team:Wageningen UR/Protocol

From 2012.igem.org

(Difference between revisions)
(→Protocol)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
{{Template:WUR}}
{{Template:WUR}}
 +
 +
<html>
 +
<script>
 +
document.getElementById("header_slider").style.backgroundImage = "url(https://static.igem.org/mediawiki/2012/a/a3/HeaderNotebook.jpg)";
 +
</script>
 +
</html>
= Methods =
= Methods =
Line 24: Line 30:
<li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li>
<li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li>
<li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li>
<li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li>
 +
<li>[[Team:Wageningen_UR/Protocol/FormationBufferPolero|Formation Buffer Polero]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>
Line 33: Line 40:
<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li>
-
<li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifing the VLPs]]</li>
+
<li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifying the VLPs]]</li>
</ul>
</ul>
Line 41: Line 48:
<li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li>
-
<li>[[Team:Wageningen_UR/Protocol/RoundupHepB|Purifing the VLPs]]</li>
+
<li>[[Team:Wageningen_UR/Protocol/RoundupHepB|Purifying the VLPs]]</li>
</ul>
</ul>
-
== TuYV Coat Protein VLP formation ==
+
== Polerovirus Coat Protein VLP formation ==
-
 
+
-
== PLRV Coat Protein VLP formation ==
+
<ul>
<ul>
<li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li>
<li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li>
 +
<li>[[Team:Wageningen_UR/Protocol/StartupPolero|Growing culture]]</li>
 +
<li>[[Team:Wageningen_UR/Protocol/DialysisPolero|Dialysis of the VLPs]]</li>
 +
<li>[[Team:Wageningen_UR/Protocol/RoundupPolero|Purifying the VLPs]]</li>
</ul>
</ul>
Line 60: Line 68:
<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li>
<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li>
<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li>
<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li>
 +
<li>[[Team:Wageningen_UR/Protocol/Mutagenesis|Mutagenesis]]</li>
</ul>
</ul>

Latest revision as of 10:44, 26 October 2012

Contents

Methods

The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.

Protocols

Medium & Buffer recipes

CCMV Coat Protein VLP formation

Hepatitis B Coat Protein VLP formation

Polerovirus Coat Protein VLP formation

General Protocol

Retrieved from "http://2012.igem.org/Team:Wageningen_UR/Protocol"