Team:Wageningen UR/Protocol

From 2012.igem.org

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= Methods =
= Methods =
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<ul>
<ul>
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<li>The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. [[Team:Wageningen_UR/MethodsProduction|Click here to read more]]</li>
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<li>[[Team:Wageningen_UR/MethodsProduction|Production]]</li>
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<li>After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. [[Team:Wageningen_UR/MethodsPurification|Click here to read more]]</li>
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<li>[[Team:Wageningen_UR/MethodsPurification|Purification]]</li>
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= Protocol =
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= Protocols =
== Medium & Buffer recipes ==
== Medium & Buffer recipes ==
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<li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li>
<li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li>
<li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li>
<li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li>
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<li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li>
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<li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li>
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<li>[[Team:Wageningen_UR/Protocol/FormationBufferPolero|Formation Buffer Polero]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>
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<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifing the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifying the VLPs]]</li>
</ul>
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<li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li>
<li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupHepB|Purifing the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupHepB|Purifying the VLPs]]</li>
</ul>
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== TuYV Coat Protein VLP formation ==
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== Polerovirus Coat Protein VLP formation ==
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== PLRV Coat Protein VLP formation ==
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<ul>
<ul>
<li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li>
<li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li>
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<li>[[Team:Wageningen_UR/Protocol/StartupPolero|Growing culture]]</li>
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<li>[[Team:Wageningen_UR/Protocol/DialysisPolero|Dialysis of the VLPs]]</li>
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<li>[[Team:Wageningen_UR/Protocol/RoundupPolero|Purifying the VLPs]]</li>
</ul>
</ul>
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<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li>
<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li>
<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li>
<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li>
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<li>[[Team:Wageningen_UR/Protocol/Mutagenesis|Mutagenesis]]</li>
</ul>
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Latest revision as of 10:44, 26 October 2012

Contents

Methods

The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.

Protocols

Medium & Buffer recipes

CCMV Coat Protein VLP formation

Hepatitis B Coat Protein VLP formation

Polerovirus Coat Protein VLP formation

General Protocol

Retrieved from "http://2012.igem.org/Team:Wageningen_UR/Protocol"