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Team:Wageningen UR/Protocol
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| + | |||
| + | = Methods = | ||
| + | |||
| + | The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together. | ||
| + | |||
| + | <ul> | ||
| + | <li>[[Team:Wageningen_UR/MethodsProduction|Production]]</li> | ||
| + | <li>[[Team:Wageningen_UR/MethodsPurification|Purification]]</li> | ||
| + | </ul> | ||
| + | |||
| + | = Protocols = | ||
== Medium & Buffer recipes == | == Medium & Buffer recipes == | ||
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<li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li> | <li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li> | <li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/FormationBufferPolero|Formation Buffer Polero]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li> | <li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li> | <li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li> | ||
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<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li> | <li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li> | <li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li> | ||
| - | <li>[[Team:Wageningen_UR/Protocol/RoundupCCMV| | + | <li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifying the VLPs]]</li> |
</ul> | </ul> | ||
== Hepatitis B Coat Protein VLP formation == | == Hepatitis B Coat Protein VLP formation == | ||
| - | + | <ul> | |
| + | <li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/RoundupHepB|Purifying the VLPs]]</li> | ||
| + | </ul> | ||
| - | == | + | == Polerovirus Coat Protein VLP formation == |
<ul> | <ul> | ||
| - | <li>[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li> | + | <li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li> |
| + | <li>[[Team:Wageningen_UR/Protocol/StartupPolero|Growing culture]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/DialysisPolero|Dialysis of the VLPs]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/RoundupPolero|Purifying the VLPs]]</li> | ||
</ul> | </ul> | ||
| Line 43: | Line 68: | ||
<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li> | <li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li> | <li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/Mutagenesis|Mutagenesis]]</li> | ||
</ul> | </ul> | ||
Latest revision as of 10:44, 26 October 2012
Contents |
Methods
The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.
Protocols
Medium & Buffer recipes
- LB medium
- SOB medium
- SOC medium
- Disassembly buffer
- Dialysis buffer 10x
- Reassembly buffer
- Virus buffer
- Formation Buffer HepBcAg
- Washing Buffer HepBcAg
- Formation Buffer Polero
- Towbin's electrotransfer buffer 1x
- Towbin's electrotransfer buffer 10x
CCMV Coat Protein VLP formation
Hepatitis B Coat Protein VLP formation
Polerovirus Coat Protein VLP formation
General Protocol
- Preparation of electrocompetent cells of E. coli
- SDS-Polyacrylamide and native gel electrophoresis
- Dynamic Light Scattering user manual
- French press user manual
- FPLC user manual
- Mutagenesis
