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                     <nav>
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                                   <li><a href="/wiki/index.php?title=Template:SUSTC-B1&action=edit">Edit</a></li>
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                                   <li><a href="/wiki/index.php?title=Template:SUSTC-B1&action=history">History</a></li>
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                                   <li><a href="/Special:MovePage/Template:SUSTC-B1">Move</a></li>
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                              </ul>
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                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/October">October</a></li>
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                               <div class="slidedesc">真っ赤に泣き腫らした瞳</div>
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                               <div class="slidedesc">South University of Science and Technology of China.</div>
                           </div>
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                               <div class="slidedesc">二人きりで酔いしれる　恋のワインに</div>
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                               <div class="slidedesc">2012 SUSTC IGEM team members.</div>
                           </div>
                           </div>
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                               <img src="https://static.igem.org/mediawiki/2012/archive/7/75/20121022104002%21Slider.3.JPG" alt="" class="change" />
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                               <div class="slidedesc">ただ見つめてふいにキス　恋の魔法</div>
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                               <div class="slidedesc">Web Page of Transcriptional Terminator Efficiency Calculator.</div>
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                           </div>
                           <div class="bigimg">
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                               <div class="slidedesc">Lab Work.</div>
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                               <div class="slidedesc">二人包む星たちの　優しい旋律</div>  
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                               <div class="slidedesc">Human Practice.</div>  
                           </div>
                           </div>
                           <div class="bigimg">
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                               <div class="slidedesc">なんて甘い恋の罠</div>
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                               <div class="slidedesc">HK jamboree</div>
                           </div>
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                                <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
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<h1>Welcome</h1>
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<p>We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.</p>
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<p> We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.</p>
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<p> To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.</p>
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<p>Nam eget justo velit. Vestibulum dignissim vestibulum volutpat. Morbi quam odio, facilisis ut scelerisque id, pretium blandit diam. Suspendisse quam magna, sollicitudin vitae volutpat et, porttitor ut sem. Mauris nec dolor erat. Vestibulum condimentum libero ac elit rutrum pharetra. Nunc aliquet nisi et erat vulputate pellentesque.</p>
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<p> We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.</p>
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Latest revision as of 15:07, 25 October 2012

Title



Transcriptional Terminator Efficiency Calculator (TTEC)

We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.

We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.

To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.

We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.

South University of Science and Technology of China

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