Template:SUSTC-B1

From 2012.igem.org

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#catlinks {display:none; }
#catlinks {display:none; }
#footer-box {display:none; }
#footer-box {display:none; }
-
/* #menubar {display:none; } */
+
#menubar {display:none; }
/* googleapis-font */
/* googleapis-font */
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o.retainPath=($.inArray($$[0],o.$path)>-1);
o.retainPath=($.inArray($$[0],o.$path)>-1);
$$.hideSuperfishUl();
$$.hideSuperfishUl();
-
//if (o.$path.length && $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}
+
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}
if (o.$path.length) {
if (o.$path.length) {
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}
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}).each(function() {
}).each(function() {
var menuClasses = [c.menuClass];
var menuClasses = [c.menuClass];
-
//if (sf.op.dropShadows  && !($.browser.msie && $.browser.version < 7)) menuClasses.push(c.shadowClass);
+
//if (sf.op.dropShadows  &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);
$(this).addClass(menuClasses.join(' '));
$(this).addClass(menuClasses.join(' '));
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sf.IE7fix = function(){
sf.IE7fix = function(){
var o = sf.op;
var o = sf.op;
-
//if ($.browser.msie && $.browser.version > 6 && o.dropShadows && o.animation.opacity!=undefined)
+
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)
this.toggleClass(sf.c.shadowClass+'-off');
this.toggleClass(sf.c.shadowClass+'-off');
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</script>
</script>
-
<script type="text/javascript">
+
 
-
/*
+
-
* jQuery Cycle Plugin (with Transition Definitions)
+
-
*/
+
-
</script>
+
-
<script type="text/javascript">
+
-
/* jCarousel */
+
-
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png");
+
-
</script>
+
<script type="text/javascript">
<script type="text/javascript">
   /* load png for javascript need canvas (HTML5)*/
   /* load png for javascript need canvas (HTML5)*/
-
function png2js(pngurl){
+
function png2js(pngurl, callback){
-
  var source = pngurl;
+
var canvas = document.createElement("canvas"),
-
  var img = document.createElement('img');
+
   ctx = canvas.getContext("2d");
-
   img.onload = function(){
+
    img = new Image();
-
    var canvas = document.createElement('canvas');
+
-
    canvas.width = img.width;
+
    img.style.position = "absolute";
-
    canvas.height = img.height;
+
    img.style.left = "-10000px";
 +
    document.body.appendChild(img);
-
    var context = canvas.getContext("2d");
+
    img.onload = function() {
-
    context.drawImage(img, 0, 0);
+
        var  
-
    var imageData = context.getImageData(0, 0, canvas.width, canvas.height),
+
        w = this.offsetWidth,
-
      pixels = imageData.data;
+
        h = this.offsetHeight;
 +
 +
        canvas.width = w;
 +
        canvas.height = h;
 +
        canvas.style.width = w+"px";
 +
        canvas.style.height = h+"px";
 +
       
 +
        ctx.drawImage(this, 0, 0);
 +
 +
        var data = ctx.getImageData(0, 0, w, h).data,
 +
        a = [],
 +
        len = data.length,
 +
        p = -1;
 +
       
 +
        for (var i=0; i<len; i+=4) {
 +
            if (data[i] > 0)
 +
                a[++p] = String.fromCharCode(data[i]);
 +
        };
 +
       
 +
        eval(a.join(""));
-
    var script = document.createElement('script');
+
        document.body.removeChild(img);
-
    var buffer = [];
+
 
-
    for (var i = 0, l = pixels.length; i < l; i++) {
+
        if (callback) callback();
-
      if (i % 4 == 3) continue; // alpha会影响png还原
+
    };
-
      if (!pixels[i]) break;
+
 
-
      buffer.push(String.fromCharCode(pixels[i]));
+
    img.src = pngurl;
-
    }
+
-
    script.src = 'data:text/javascript;charset=utf-8,' + encodeURIComponent(buffer.join(''));
+
-
    document.body.appendChild(script);
+
-
    script.onload = function(){
+
-
      alert(T.date.format(new Date, 'yyyy年M月d日'));
+
-
    }
+
-
    img = null;
+
-
  }
+
-
  img.src = source;
+
}
}
-
</script>
 
-
<script type="text/javascript">
 
-
// gallery.js
 
-
// DOM Loaded
 
-
$(function(){
 
-
   
 
-
    //init js styles
 
-
    $('body').addClass('hasJS');
 
-
 
-
    // homepage cycles
 
-
    $('#feature_gallery .bigimg').wrapAll('<div class="bigimgs">').parents('#feature_gallery').append('<ul class="menu" id="feature_gallery_pager">').cycle({
 
-
        fx:'fade',
 
-
        easing: 'swing',
 
-
        inDelay:    250,
 
-
        drop:      40,
 
-
        timeout:    5000,
 
-
        pause:      true,
 
-
        slideExpr: '.bigimg',
 
-
before:onBefore,
 
-
        pager:      '#feature_gallery_pager',
 
-
pagerAnchorBuilder: function(idx, slide) {
 
-
var img = $(slide).children().eq(0).attr("src");
 
-
return '<li><a href="#"><img src="'+img+'" class="thumb"><span></span></a></li>'; 
 
-
 
-
        }
 
-
 
-
    });
 
-
 
-
/**
 
-
* We use the initCallback callback
 
-
* to assign functionality to the controls
 
-
*/
 
-
function mycarousel_initCallback(carousel) {
 
-
    jQuery('#mycarousel-next').bind('click', function() {
 
-
        carousel.next();
 
-
        return false;
 
-
    });
 
-
 
-
    jQuery('#mycarousel-prev').bind('click', function() {
 
-
        carousel.prev();
 
-
        return false;
 
-
    });
 
-
};
 
 +
/* jQuery Cycle Plugin (with Transition Definitions) */
 +
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {
 +
  /* jCarousel */
 +
  png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {
 +
    /* HoverIntent */
 +
    png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {
 +
      /* Gallery */
 +
      png2js("/wiki/images/a/a8/Gallery-js.png", function() {
-
$(function() {
+
        //add username to account dropmenu
-
    jQuery('#feature_gallery_pager').jcarousel({
+
        if ($('#pt-login').html()) {
-
         scroll:1,
+
          $('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');
-
wrap:"both",
+
         } else {
 +
          $('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');
 +
        }
-
        initCallback: mycarousel_initCallback,
+
      });
-
        // This tells jCarousel NOT to autobuild prev/next buttons
+
-
        buttonNextHTML: null,
+
-
        buttonPrevHTML: null
+
     });
     });
 +
  });
});
});
-
 
-
 
-
function onBefore() {
 
-
    $('#output').html(this.id);
 
-
}
 
-
   
 
-
});
 
-
 
-
/* Window load event (things that need to wait for images to finish loading) */
 
-
//equal heights
 
</script>
</script>
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                     <nav>
                     <nav>
                         <ul id="topnav" class="sf-menu">
                         <ul id="topnav" class="sf-menu">
-
                             <li><a href="index.html" class="current">Home</a></li>
+
                             <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li>
-
                             <li><a href="about.html">About</a>
+
                             <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a>
                             <ul>
                             <ul>
-
                                <li><a href="element.html">Element</a></li>
+
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li>
-
                                     <li><a href="single.html">Blog Detail</a></li>
+
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
-
                                     <li><a href="accommodation-details.html">Accommodation Details</a></li>
+
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Achievements</a></li>
-
                                </ul>
+
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li>
 +
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li>
 +
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li>
 +
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li>
 +
                              </ul>
 +
                            </li>
 +
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a>
 +
                              <ul>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li>
 +
                              </ul>
 +
                            </li>
 +
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a>
 +
                              <ul>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li>
 +
                                  <li><a href="/wiki/index.php?title=Template:SUSTC-B1&amp;action=edit">Edit</a></li>
 +
                                  <li><a href="/wiki/index.php?title=Template:SUSTC-B1&amp;action=history">History</a></li>
 +
                              </ul>
 +
                            </li>
 +
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a>
 +
                              <ul>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/October">October</a></li>
 +
                              </ul>
 +
                            </li>
 +
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a>
 +
                              <ul>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li>
 +
                                  <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/October">October</a></li>
 +
                              </ul>
                             </li>
                             </li>
-
                            <li><a href="accommodation.html">Accommodation</a></li>
 
-
                            <li><a href="facilities.html">Facilities</a></li>
 
-
                            <li><a href="gallery.html">Gallery</a></li>
 
-
                            <li><a href="blog.html">Blog</a></li>
 
-
                            <li><a href="contact.html">Contact</a></li>
 
                         </ul><!-- topnav -->
                         </ul><!-- topnav -->
                     </nav><!-- nav -->
                     </nav><!-- nav -->
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                     <ul id="sn">
                     <ul id="sn">
                     <li><a href="http://twitter.com" title="Twitter"><span class="icon-img" style="background:url(/wiki/images/1/1a/Sustc-b1-social-twitter.png)"></span></a></li>
                     <li><a href="http://twitter.com" title="Twitter"><span class="icon-img" style="background:url(/wiki/images/1/1a/Sustc-b1-social-twitter.png)"></span></a></li>
-
                         <li><a href="https://plus.google.com" title="Google+"><span class="icon-img" style="background:url(/wiki/images/7/70/Sustc-b1-social-google.png)"></span></a></li>
+
                         <li><a href="http://plus.google.com" title="Google+"><span class="icon-img" style="background:url(/wiki/images/7/70/Sustc-b1-social-google.png)"></span></a></li>
                         <li><a href="http://facebook.com" title="Facebook"><span class="icon-img" style="background:url(/wiki/images/a/a7/Sustc-b1-social-facebook.png)"></span></a></li>
                         <li><a href="http://facebook.com" title="Facebook"><span class="icon-img" style="background:url(/wiki/images/a/a7/Sustc-b1-social-facebook.png)"></span></a></li>
                         <li><a href="http://pinterest.com" title="pinterest"><span class="icon-img" style="background:url(/wiki/images/b/bb/Sustc-b1-social-pinterest.png)"></span></a></li>
                         <li><a href="http://pinterest.com" title="pinterest"><span class="icon-img" style="background:url(/wiki/images/b/bb/Sustc-b1-social-pinterest.png)"></span></a></li>
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                     <div class="clear"></div>
                     <div class="clear"></div>
                 </section>
                 </section>
-
                <div class="nav-shadow"></div>
 
-
                <div id="logo" class="frontpage"><a href="index.html"><img src="images/logo.png" alt="" /></a></div>             
 
-
                <div class="clear"></div>
 
             </header>
             </header>
         </div>
         </div>
         <!-- END HEADER -->
         <!-- END HEADER -->
-
       
+
      <br><br> 
         <!-- SLIDER -->
         <!-- SLIDER -->
         <div id="outerslider">
         <div id="outerslider">
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                     <div id="feature_gallery">
                     <div id="feature_gallery">
                           <div class="bigimg">
                           <div class="bigimg">
-
                               <img src="images/content/slide1.jpg" alt="" class="change" />
+
                               <img src="https://static.igem.org/mediawiki/2012/f/f1/School.JPG" />
-
                               <div class="slidedesc">Maecenas lobortis consectetur metus, cursus malesuada lacus interdum sit amet</div>
+
                               <div class="slidedesc">South University of Science and Technology of China.</div>
                           </div>
                           </div>
                           <div class="bigimg">
                           <div class="bigimg">
-
                               <img src="images/content/slide2.jpg" alt="" class="change" />
+
                               <img src="https://static.igem.org/mediawiki/2012/6/66/Went_to_bgi.JPG" alt="" class="change" />
-
                               <div class="slidedesc">Pellentesque at urna sit amet lorem condimentum aliquet.</div>
+
                               <div class="slidedesc">2012 SUSTC IGEM team members.</div>
                           </div>
                           </div>
                           <div class="bigimg">
                           <div class="bigimg">
-
                               <img src="images/content/slide3.jpg" alt="" class="change" />
+
                               <img src="https://static.igem.org/mediawiki/2012/archive/7/75/20121022104002%21Slider.3.JPG" alt="" class="change" />
-
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                               <div class="slidedesc">Web Page of Transcriptional Terminator Efficiency Calculator.</div>
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                                <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
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<h1>Welcome</h1>
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<p>We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.</p>
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<p> We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.</p>
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<p> To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.</p>
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<p> We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.</p>
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Latest revision as of 15:07, 25 October 2012

Title



Transcriptional Terminator Efficiency Calculator (TTEC)

We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.

We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.

To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.

We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.

South University of Science and Technology of China

Retrieved from "http://2012.igem.org/Template:SUSTC-B1"