From 2012.igem.org

Latest revision as of 13:30, 23 October 2012

BioBricks

BioBricks constructed during iGEM 2012

Overview for TU Munich's iGEM project 2012

List of all Parts

Experiences with existing BioBricks

Rating working BioBricks: [http://partsregistry.org/Part:BBa_K300007 BBa_K300007]

[http://partsregistry.org/Part:BBa_K300007:Experience Yeast Integration Vector BBa_K300007] (Partly showed functionality)

Identification of incorrect BioBricks: [http://partsregistry.org/Part:BBa_K268000 BBa_K268000]

[http://partsregistry.org/Part:BBa_K268000:Experience#User_Reviews| Forbidden restriction sites in pSB6A0 (BBa_K268000g)]

Improvement of an existing BioBrick: [http://partsregistry.org/Part:BBa_K801100 BBa_K801100]

In 2012 the [http://partsregistry.org Registry of Standard Biological Parts] defined <partinfo>BBa_J04450</partinfo> as the standard shipping part that is required for submission of backbones, creating the need for a RFC25 compatible standard shipping part.

We extended the standard compability of the RFP coding device (<partinfo>BBa_J04450</partinfo>) to RFC10 and [http://hdl.handle.net/1721.1/45140 RFC25] by adding the NgoMIV and AgeI restriction sites into the prefix and suffix of this part. Additionally two AgeI restriction sites that were present in the generator itself were deleted.

This part may be used as a standard insert for RFC10 as well as [http://hdl.handle.net/1721.1/45140 RFC25] backbones. This improvement became necessary because insertion of <partinfo>BBa_J04450</partinfo> into a RFC25 compatible backbone leads to the deletion of the desired [http://hdl.handle.net/1721.1/45140 RFC25] restriction sites that are needed for protein fusions.