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Revision as of 11:44, 22 October 2012

Title



Transcriptional Terminator Efficiency Calculator (TTEC)

We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.

We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.

To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.

We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.

1

Design Ideas

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

2

Problem Areas

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

3

Green Garden

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

4

Planting Areas

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

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