Team:Berkeley/Parts

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<b>Submitted parts:</b> <br><br>
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<b>Submitted parts:</b> <br>
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1 Nucleus targeting Protein <br>
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1 Nucleus targeting protein- Registry Part:<a href="http://partsregistry.org/Part:BBa_K900002">BBa_K900002</a> <br>
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1 Actin targeting protein <br>
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1 Vacuolar membrane targeting protein- Registry Part:<a href="http://partsregistry.org/Part:BBa_K900003">BBa_K900003</a> <br>  
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1 Vacuolar membrane targeting protein <br>
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1 Actin targeting protein- Registry Part:<a href="http://partsregistry.org/Part:BBa_K900004">BBa_K900004</a> <br>  
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1 Cellular periphery targeting sequence <br>
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1 Plasma membrane targeting sequence- Registry Part:<a href="http://partsregistry.org/Part:BBa_K900005">BBa_K900005</a><br>
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2 Photoactivatable fluorescent proteins (red and green) <br>
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2 Photoactivatable fluorescent proteins (red-PAmCHerry and green-PAGFP)-
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<b> Total: 6 parts submitted to registry </b><br><br>
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Registry Parts: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K900000">BBa_K900000</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K900001">BBa_K900001</a><br>
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The parts we constructed for registry submission may be viewed <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=Berkeley">here</a>.
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We also built and characterized 3 promoters from the registry (<a href="https://2012.igem.org/Team:Berkeley/Project/partsregistry.org/wiki/index.php?title=Part:BBa_I766557">pSTE5</a>, <a href="https://2012.igem.org/Team:Berkeley/Project/partsregistry.org/wiki/index.php?title=Part:BBa_I766555">pCYC1</a>, and <a href="https://2012.igem.org/Team:Berkeley/Project/partsregistry.org/wiki/index.php?title=Part:BBa_I766556">pADH1</a>), comparing them to another promoter (pTDH3) that we used. The data comparing the relative promoter strengths can be found <a href="https://static.igem.org/mediawiki/2012/0/0f/IGEM_promoter_characterization.png">here</a>.
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<b> Total: 6 parts submitted to registry </b><br><br>
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The entirety of the registry submission can be found <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=Berkeley">here</a>.
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We also built and characterized 4 promoters from the registry, pSTE5, pCYC1, pADH1, and pTDH3 (Registry Parts: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I766557">BBa_I766557</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I766555">BBa_I766555</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I766556">BBa_I766556</a>, <a href="http://partsregistry.org/Part:BBa_K124002">BBa_K124002</a>). The experimental method and data concerning the experiment can be found <a href="https://2012.igem.org/Team:Berkeley/Project/Localization#Promoter Characterization">here</a> and in the Experiences subsection for each part.
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Latest revision as of 03:51, 16 October 2012

header
iGEM Berkeley iGEMBerkeley iGEMBerkeley

Submitted parts:
1 Nucleus targeting protein- Registry Part:BBa_K900002
1 Vacuolar membrane targeting protein- Registry Part:BBa_K900003
1 Actin targeting protein- Registry Part:BBa_K900004
1 Plasma membrane targeting sequence- Registry Part:BBa_K900005
2 Photoactivatable fluorescent proteins (red-PAmCHerry and green-PAGFP)- Registry Parts: BBa_K900000, BBa_K900001
Total: 6 parts submitted to registry

The entirety of the registry submission can be found here.

We also built and characterized 4 promoters from the registry, pSTE5, pCYC1, pADH1, and pTDH3 (Registry Parts: BBa_I766557, BBa_I766555, BBa_I766556, BBa_K124002). The experimental method and data concerning the experiment can be found here and in the Experiences subsection for each part.