"
Team:uOttawa CA/Results
From 2012.igem.org
(Difference between revisions)
| (13 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
<html> | <html> | ||
<a name="top"></a> | <a name="top"></a> | ||
| - | <a href="#scroll-one"> | + | <table class="center bold" width="970"> |
| - | <a href="#top"><img src="https://static.igem.org/mediawiki/2012/ | + | <tr> |
| + | <td><a href="#scroll-one" class="Orange">Analysis</a></td> | ||
| + | <td><a href="#scroll-two" class="Orange">Promoters</a></td> | ||
| + | <td><a href="#scroll-three" class="Orange">Protocols</a></td> | ||
| + | </tr> | ||
| + | </table><br /><br /> | ||
| + | |||
| + | <html> | ||
| + | <a name="scroll-one"></a> | ||
| + | <a href="#top"><img src="https://static.igem.org/mediawiki/2012/e/e7/UottawaArrowtotheknee6_out.png" alt="" /></a> | ||
</html> | </html> | ||
| - | + | ||
| + | '''Characterization Data''' | ||
<html> | <html> | ||
<a href="https://static.igem.org/mediawiki/2012/5/51/UottawaResults2.png" target="_blank"><img class="center" width="300" src="https://static.igem.org/mediawiki/2012/5/51/UottawaResults2.png" /></a> | <a href="https://static.igem.org/mediawiki/2012/5/51/UottawaResults2.png" target="_blank"><img class="center" width="300" src="https://static.igem.org/mediawiki/2012/5/51/UottawaResults2.png" /></a> | ||
| - | </html><br /> | + | </html><br /><br /> |
The strains for characterizing the Tet repressor have been built. Characterization data of the Tet-BFP diploid strains (b) are shown below. | The strains for characterizing the Tet repressor have been built. Characterization data of the Tet-BFP diploid strains (b) are shown below. | ||
| - | |||
| - | |||
| - | |||
<html> | <html> | ||
| - | <a href="https://static.igem.org/mediawiki/2012/ | + | <a href="https://static.igem.org/mediawiki/2012/3/33/UottawaResultsFINAL2.png" target="_blank"><img class="center" width="450" src="https://static.igem.org/mediawiki/2012/3/33/UottawaResultsFINAL2.png" /></a> |
| - | </html><br /><br /><br /> | + | </html><br /><br /><br /> |
| - | + | ||
<html> | <html> | ||
| - | <a | + | <a name="scroll-two"></a> |
| - | </html | + | <a href="#top"><img src="https://static.igem.org/mediawiki/2012/9/9f/UottawaArrowtotheknee3.png" alt="" /></a> |
| + | </html> | ||
| - | + | '''Promoter Design''' | |
| - | + | ||
| - | + | ||
| + | To test our promoter design protocol, we decided to use the vector PRS416 and primers comprising the distal region of the GAL1 promoter with GAL4 binding sites replaced with tet operator sites. We submitted 9 plasmids for sequencing and our results below elucidate the sequence of our GAL-1 promoter within the PRS416 plasmid. | ||
| + | <center><html> | ||
| + | <a href="https://static.igem.org/mediawiki/2012/2/28/UottawaResults8.png" target="_blank"><img class="middle" width="800" src="https://static.igem.org/mediawiki/2012/2/28/UottawaResults8.png" /></a> | ||
| + | </html></center><br /> | ||
| + | <center>Of the 9 plasmids sequenced, 3 plasmids contained the desired sequence. Thus as a preliminary estimate, our efficiency is about 33%. | ||
<html> | <html> | ||
| - | <a | + | <a href="https://static.igem.org/mediawiki/2012/8/86/UottawaResults9.png" target="_blank"><img class="middle" width="450" src="https://static.igem.org/mediawiki/2012/8/86/UottawaResults9.png" /></a> |
| - | + | ||
</html> | </html> | ||
| + | <br /><br /> | ||
| + | <html> | ||
| + | <a name="scroll-three"></a> | ||
| + | <a href="#top"><img src="https://static.igem.org/mediawiki/2012/4/48/UottawaArrowtotheknee5_out.png" alt="" /></a> | ||
| + | </html></center> | ||
| - | + | <html> | |
| + | <a href="https://static.igem.org/mediawiki/2012/3/38/UottawaPROTOCOL1A.png" target="_blank"><img class="fl" width="450" src="https://static.igem.org/mediawiki/2012/3/38/UottawaPROTOCOL1A.png" /></a> | ||
| + | </html> | ||
| + | |||
| + | <html> | ||
| + | <a href="https://static.igem.org/mediawiki/2012/5/59/UottawaPROTOCOL1B.png" target="_blank"><img class="fr" width="450" src="https://static.igem.org/mediawiki/2012/5/59/UottawaPROTOCOL1B.png" /></a> | ||
| + | |||
| + | <html> | ||
| + | <a href="https://static.igem.org/mediawiki/2012/6/62/UottawaDNA1.png" target="_blank"><img class="fl" width="450" src="https://static.igem.org/mediawiki/2012/6/62/UottawaDNA1.png" /></a> | ||
| + | </html> | ||
| - | |||
{{Template:uOttawa_Footer}} | {{Template:uOttawa_Footer}} | ||
Latest revision as of 03:58, 4 October 2012
 |
 |
 |
 |
 |
 |
 |
 |
| Analysis | Promoters | Protocols |
Characterization Data
The strains for characterizing the Tet repressor have been built. Characterization data of the Tet-BFP diploid strains (b) are shown below.
Promoter Design
To test our promoter design protocol, we decided to use the vector PRS416 and primers comprising the distal region of the GAL1 promoter with GAL4 binding sites replaced with tet operator sites. We submitted 9 plasmids for sequencing and our results below elucidate the sequence of our GAL-1 promoter within the PRS416 plasmid.
