Team:Nevada/Week 12

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Revision as of 03:55, 4 October 2012



Protocols | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 |


Week 12: August 6 - August 10

Contents

August 6

Joe: colony check SBP-RFP + terminator
Michelle:
Check transformation of SBP (SpeI and PstI)-LRP (PstI and XbaI) digested with XbaI
and PstI and CP-EP* L-arabinose (SpeI and NSI).

Miniprep SBP (SpeI and PstI)-LRP (PstI and XbaI) and digest with SpeI and PstI.

Jeremiah & Chris:
Colony PCR done to check if the terminator added successfully (gel #133)
Grow colony 12
Digest TBP+ again incase colony 12 fails


August 7

Joe: miniprep SBP-RFP-Terminator
digest by EcoRI/PstI to check, digest by EcoRI/ XbaI for ligation
Gel 139- both digestions good
Ligate SBP-RFP-Terminatior and promoter
Transform, plate on Kana
Michelle:
Run gel to check digest SBP (SpeI and PstI)-LRP (PstI and XbaI) with SpeI and PstI,
heat inactivate, and dephosphorylate digest.
Ligate SBP (SpeI and PstI)-LRP (PstI and XbaI) with SpeI and PstI digest with
Terminator (PstI and XbaI) digest followed by transformation into TOP 10 competent cells onto KAN plates.
Justin and Dafne
Clean corn starch 3x
Place 200 ul of refolded protein in 1mg/ml solution of corn starch in 1.5 ml epindorf tube

August 8

August 9

==August 10==