Team:Cornell/testing/notebook/drylab/8

From 2012.igem.org

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<h6>Dry Lab</h6>
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<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/pre">Pre</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/1">Week 1</a>
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<h3>Thursday, July 26, 2012</h3>
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It has been said that <span class="has-tip" data-width="250" title="Description of what astronomy is.">astronomy</span> is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known. <a href="#" class="technical-desc" for="#technical-desc1" style="display:block;margin-top:20px;">Technical Notes</a>
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Focus: Updates on Inventory, Bioreactor Production<p></p>
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<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc1">
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<h6>Technical Stuff:</h6>
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Watch out for children under 5.
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<a href="#" class="technical-desc" for="#technical-desc4" style="display:block;margin-top:20px;">Details</a>
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<h3>Monday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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<a href="#" class="technical-desc" for="#technical-desc7" style="display:block;margin-top:20px;">Technical Notes</a>
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<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc7">
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<h6>Technical Stuff:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Tuesday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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<a href="#" class="technical-desc" for="#technical-desc2" style="display:block;margin-top:20px;">Technical Notes</a>
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<h6>Technical Stuff:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Wednesday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
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<h6>Entry:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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Yesterday, we picked up the Pelican 1650 at the shipping storage room. When we took it out of the box, its large, black, chiseled surfaces and side locks made it look more intimidating face to face. A very observant Manny pointed out the lifetime guarantee on the box, which stated the case is built to withstand everything except for shark bites, bear attacks, and children under 5. We had a few good laughs there before we looked through the rest of our inventory: the polycarbonate tube and DelRen rod that were initially planned to make the bioreactor shell; the graphite rods, luer fittings from Cole-Palmer, and a free sample of septas from Restek for the bioreactor; and the valves and tubes for the micropumps.<p></p>
 +
We were also going to start machining yesterday. However, when we arrived at the Emerson machine shop, we found out that it was closed until August 6th for maintenance, which drastically pushes back our schedule for production. Chie and Tina left, while the rest of us went to try the machine shop at Clark Hall. To work there, we need to take a 24 hour training course in order to use those machines, so we decided to pay the shop to machine the bioreactor. That way, Dylan can start running tests in the new bioreactor as soon as possible. We will machine another three bioreactors ourselves once the Emerson shop reopens.<p></p>
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#chassis #bioreactor
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<h3>Friday</h3>
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<h3>Friday, July 27, 2012</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
Focus: Autoclavability of the Bioreactor Design, Filtration System<p></p>
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<a href="#" class="technical-desc" for="#technical-desc5" style="display:block;margin-top:20px;">Technical Notes</a>
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 +
Orders just in, leave no bacteria alive.
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<a href="#" class="technical-desc" for="#technical-desc5" style="display:block;margin-top:20px;">Details</a>
<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc5">
<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc5">
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<h6>Technical Stuff:</h6>
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<h6>Entry:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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Dan decided to scrap the solar panel casing. According to Bruce Land, it would be unnecessary because solar panels are built operate outdoors for around 20 years. Also, we considered autoclave temperatures versus the maximum temperatures for polycarbonate and DelRen. Polycarbonate reaches its temperature limit in the autoclave, while DelRen cannot withstand temperatures as nearly high as 250 F. Depending on how the first bioreactor turns on, we may switch the bioreactor shell materials to teflon and glass. <p></p>
 +
Maneesh and Dan also looked at filtration systems. Although we ordered hollow fiber module filter for the influent channel, we need to decide what to do for the effluent. Assuming all shewanella fall off the rods per hour, ~8000 mm3 of bacteria would accumulate in the effluent channel in 6 months. <p></p>
 +
Lydia continued working with the microcontroller so that it sends an oscillating signal to the micropump. Bruce suggested that she read the manual first to figure out which pins on the chip are best to relay the signals. Kelvin finished the server and started working on the server to client transaction.<p></p>
 +
 
 +
#solar panel #filtration #electronics #software
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<div class="three columns">
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<h3>Saturday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
-
<a href="#" class="technical-desc" for="#technical-desc6" style="display:block;margin-top:20px;">Technical Notes</a>
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<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc6">
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<h6>Technical Stuff:</h6>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Thursday, July 26, 2012</h3>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
Yesterday, we picked up the Pelican 1650 at the shipping storage room. When we took it out of the box, its large, black, chiseled surfaces and side locks made it look more intimidating face to face. A very observant Manny pointed out the lifetime guarantee on the box, which stated the case is built to withstand everything except for shark bites, bear attacks, and children under 5. We had a few good laughs there before we looked through the rest of our inventory: the polycarbonate tube and DelRen rod that were initially planned to make the bioreactor shell; the graphite rods, luer fittings from Cole-Palmer, and a free sample of septas from Restek for the bioreactor; and the valves and tubes for the micropumps.<p></p>
 +
We were also going to start machining yesterday. However, when we arrived at the Emerson machine shop, we found out that it was closed until August 6th for maintenance, which drastically pushes back our schedule for production. Chie and Tina left, while the rest of us went to try the machine shop at Clark Hall. To work there, we need to take a 24 hour training course in order to use those machines, so we decided to pay the shop to machine the bioreactor. That way, Dylan can start running tests in the new bioreactor as soon as possible. We will machine another three bioreactors ourselves once the Emerson shop reopens.<p></p>
 +
 
 +
#chassis #bioreactor
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Saturday</h3>
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<h3>Friday, July 27, 2012</h3>
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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
Dan decided to scrap the solar panel casing. According to Bruce Land, it would be unnecessary because solar panels are built operate outdoors for around 20 years. Also, we considered autoclave temperatures versus the maximum temperatures for polycarbonate and DelRen. Polycarbonate reaches its temperature limit in the autoclave, while DelRen cannot withstand temperatures as nearly high as 250 F. Depending on how the first bioreactor turns on, we may switch the bioreactor shell materials to teflon and glass. <p></p>
 +
Maneesh and Dan also looked at filtration systems. Although we ordered hollow fiber module filter for the influent channel, we need to decide what to do for the effluent. Assuming all shewanella fall off the rods per hour, ~8000 mm3 of bacteria would accumulate in the effluent channel in 6 months. <p></p>
 +
Lydia continued working with the microcontroller so that it sends an oscillating signal to the micropump. Bruce suggested that she read the manual first to figure out which pins on the chip are best to relay the signals. Kelvin finished the server and started working on the server to client transaction.<p></p>
 +
 
 +
#solar panel #filtration #electronics #software
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<h3>Sunday</h3>
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<h3>Thursday, July 26, 2012</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
Focus: Updates on Inventory, Bioreactor Production<p></p>
-
<div class="panel" style="background:white;margin-top: 20px;">
+
 
-
<h6>Technical Stuff:</h6>
+
Watch out for children under 5.
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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<h3>Monday</h3>
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It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
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+
-
<h6>Technical Stuff:</h6>
+
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Tuesday</h3>
+
-
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Technical Stuff:</h6>
+
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Wednesday</h3>
+
-
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Technical Stuff:</h6>
+
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Thursday</h3>
+
-
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Technical Stuff:</h6>
+
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Friday</h3>
+
-
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
<div class="panel" style="background:white;margin-top: 20px;">
<div class="panel" style="background:white;margin-top: 20px;">
-
<h6>Technical Stuff:</h6>
+
<h6>Entry:</h6>
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
Yesterday, we picked up the Pelican 1650 at the shipping storage room. When we took it out of the box, its large, black, chiseled surfaces and side locks made it look more intimidating face to face. A very observant Manny pointed out the lifetime guarantee on the box, which stated the case is built to withstand everything except for shark bites, bear attacks, and children under 5. We had a few good laughs there before we looked through the rest of our inventory: the polycarbonate tube and DelRen rod that were initially planned to make the bioreactor shell; the graphite rods, luer fittings from Cole-Palmer, and a free sample of septas from Restek for the bioreactor; and the valves and tubes for the micropumps.<p></p>
 +
We were also going to start machining yesterday. However, when we arrived at the Emerson machine shop, we found out that it was closed until August 6th for maintenance, which drastically pushes back our schedule for production. Chie and Tina left, while the rest of us went to try the machine shop at Clark Hall. To work there, we need to take a 24 hour training course in order to use those machines, so we decided to pay the shop to machine the bioreactor. That way, Dylan can start running tests in the new bioreactor as soon as possible. We will machine another three bioreactors ourselves once the Emerson shop reopens.<p></p>
 +
 
 +
#chassis #bioreactor
</div>
</div>
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/Smile_square.jpg">
</div>
</div>
</div>
</div>
<div class="row last-ele">
<div class="row last-ele">
<div class="nine columns">
<div class="nine columns">
-
<h3>Saturday</h3>
+
<h3>Friday, July 27, 2012</h3>
-
It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.
+
Focus: Autoclavability of the Bioreactor Design, Filtration System<p></p>
 +
 
 +
Orders just in, leave no bacteria alive.
<div class="panel" style="background:white;margin-top: 20px;">
<div class="panel" style="background:white;margin-top: 20px;">
-
<h6>Technical Stuff:</h6>
+
<h6>Entry:</h6>
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
Dan decided to scrap the solar panel casing. According to Bruce Land, it would be unnecessary because solar panels are built operate outdoors for around 20 years. Also, we considered autoclave temperatures versus the maximum temperatures for polycarbonate and DelRen. Polycarbonate reaches its temperature limit in the autoclave, while DelRen cannot withstand temperatures as nearly high as 250 F. Depending on how the first bioreactor turns on, we may switch the bioreactor shell materials to teflon and glass. <p></p>
 +
Maneesh and Dan also looked at filtration systems. Although we ordered hollow fiber module filter for the influent channel, we need to decide what to do for the effluent. Assuming all shewanella fall off the rods per hour, ~8000 mm3 of bacteria would accumulate in the effluent channel in 6 months. <p></p>
 +
Lydia continued working with the microcontroller so that it sends an oscillating signal to the micropump. Bruce suggested that she read the manual first to figure out which pins on the chip are best to relay the signals. Kelvin finished the server and started working on the server to client transaction.<p></p>
 +
 
 +
#solar panel #filtration #electronics #software
</div>
</div>
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/Smile_square.jpg">
</div>
</div>
</div>
</div>
 +
<div class="row last-ele">
<div class="row last-ele">
<div class="three columns">
<div class="three columns">

Latest revision as of 03:34, 4 October 2012

Progress Log
Details
Both

Week 8

  • << Previous Week
    Next Week >>

    Thursday, July 26, 2012

    Focus: Updates on Inventory, Bioreactor Production

    Watch out for children under 5. Details
    Entry:
    Yesterday, we picked up the Pelican 1650 at the shipping storage room. When we took it out of the box, its large, black, chiseled surfaces and side locks made it look more intimidating face to face. A very observant Manny pointed out the lifetime guarantee on the box, which stated the case is built to withstand everything except for shark bites, bear attacks, and children under 5. We had a few good laughs there before we looked through the rest of our inventory: the polycarbonate tube and DelRen rod that were initially planned to make the bioreactor shell; the graphite rods, luer fittings from Cole-Palmer, and a free sample of septas from Restek for the bioreactor; and the valves and tubes for the micropumps.

    We were also going to start machining yesterday. However, when we arrived at the Emerson machine shop, we found out that it was closed until August 6th for maintenance, which drastically pushes back our schedule for production. Chie and Tina left, while the rest of us went to try the machine shop at Clark Hall. To work there, we need to take a 24 hour training course in order to use those machines, so we decided to pay the shop to machine the bioreactor. That way, Dylan can start running tests in the new bioreactor as soon as possible. We will machine another three bioreactors ourselves once the Emerson shop reopens.

    #chassis #bioreactor

    Friday, July 27, 2012

    Focus: Autoclavability of the Bioreactor Design, Filtration System

    Orders just in, leave no bacteria alive. Details
    Entry:
    Dan decided to scrap the solar panel casing. According to Bruce Land, it would be unnecessary because solar panels are built operate outdoors for around 20 years. Also, we considered autoclave temperatures versus the maximum temperatures for polycarbonate and DelRen. Polycarbonate reaches its temperature limit in the autoclave, while DelRen cannot withstand temperatures as nearly high as 250 F. Depending on how the first bioreactor turns on, we may switch the bioreactor shell materials to teflon and glass.

    Maneesh and Dan also looked at filtration systems. Although we ordered hollow fiber module filter for the influent channel, we need to decide what to do for the effluent. Assuming all shewanella fall off the rods per hour, ~8000 mm3 of bacteria would accumulate in the effluent channel in 6 months.

    Lydia continued working with the microcontroller so that it sends an oscillating signal to the micropump. Bruce suggested that she read the manual first to figure out which pins on the chip are best to relay the signals. Kelvin finished the server and started working on the server to client transaction.

    #solar panel #filtration #electronics #software
    << Previous Week
    Next Week >>
  • << Previous Week
    Next Week >>

    Thursday, July 26, 2012

    Yesterday, we picked up the Pelican 1650 at the shipping storage room. When we took it out of the box, its large, black, chiseled surfaces and side locks made it look more intimidating face to face. A very observant Manny pointed out the lifetime guarantee on the box, which stated the case is built to withstand everything except for shark bites, bear attacks, and children under 5. We had a few good laughs there before we looked through the rest of our inventory: the polycarbonate tube and DelRen rod that were initially planned to make the bioreactor shell; the graphite rods, luer fittings from Cole-Palmer, and a free sample of septas from Restek for the bioreactor; and the valves and tubes for the micropumps.

    We were also going to start machining yesterday. However, when we arrived at the Emerson machine shop, we found out that it was closed until August 6th for maintenance, which drastically pushes back our schedule for production. Chie and Tina left, while the rest of us went to try the machine shop at Clark Hall. To work there, we need to take a 24 hour training course in order to use those machines, so we decided to pay the shop to machine the bioreactor. That way, Dylan can start running tests in the new bioreactor as soon as possible. We will machine another three bioreactors ourselves once the Emerson shop reopens.

    #chassis #bioreactor

    Friday, July 27, 2012

    Dan decided to scrap the solar panel casing. According to Bruce Land, it would be unnecessary because solar panels are built operate outdoors for around 20 years. Also, we considered autoclave temperatures versus the maximum temperatures for polycarbonate and DelRen. Polycarbonate reaches its temperature limit in the autoclave, while DelRen cannot withstand temperatures as nearly high as 250 F. Depending on how the first bioreactor turns on, we may switch the bioreactor shell materials to teflon and glass.

    Maneesh and Dan also looked at filtration systems. Although we ordered hollow fiber module filter for the influent channel, we need to decide what to do for the effluent. Assuming all shewanella fall off the rods per hour, ~8000 mm3 of bacteria would accumulate in the effluent channel in 6 months.

    Lydia continued working with the microcontroller so that it sends an oscillating signal to the micropump. Bruce suggested that she read the manual first to figure out which pins on the chip are best to relay the signals. Kelvin finished the server and started working on the server to client transaction.

    #solar panel #filtration #electronics #software
    << Previous Week
    Next Week >>
  • << Previous Week
    Next Week >>

    Thursday, July 26, 2012

    Focus: Updates on Inventory, Bioreactor Production

    Watch out for children under 5.
    Entry:
    Yesterday, we picked up the Pelican 1650 at the shipping storage room. When we took it out of the box, its large, black, chiseled surfaces and side locks made it look more intimidating face to face. A very observant Manny pointed out the lifetime guarantee on the box, which stated the case is built to withstand everything except for shark bites, bear attacks, and children under 5. We had a few good laughs there before we looked through the rest of our inventory: the polycarbonate tube and DelRen rod that were initially planned to make the bioreactor shell; the graphite rods, luer fittings from Cole-Palmer, and a free sample of septas from Restek for the bioreactor; and the valves and tubes for the micropumps.

    We were also going to start machining yesterday. However, when we arrived at the Emerson machine shop, we found out that it was closed until August 6th for maintenance, which drastically pushes back our schedule for production. Chie and Tina left, while the rest of us went to try the machine shop at Clark Hall. To work there, we need to take a 24 hour training course in order to use those machines, so we decided to pay the shop to machine the bioreactor. That way, Dylan can start running tests in the new bioreactor as soon as possible. We will machine another three bioreactors ourselves once the Emerson shop reopens.

    #chassis #bioreactor

    Friday, July 27, 2012

    Focus: Autoclavability of the Bioreactor Design, Filtration System

    Orders just in, leave no bacteria alive.
    Entry:
    Dan decided to scrap the solar panel casing. According to Bruce Land, it would be unnecessary because solar panels are built operate outdoors for around 20 years. Also, we considered autoclave temperatures versus the maximum temperatures for polycarbonate and DelRen. Polycarbonate reaches its temperature limit in the autoclave, while DelRen cannot withstand temperatures as nearly high as 250 F. Depending on how the first bioreactor turns on, we may switch the bioreactor shell materials to teflon and glass.

    Maneesh and Dan also looked at filtration systems. Although we ordered hollow fiber module filter for the influent channel, we need to decide what to do for the effluent. Assuming all shewanella fall off the rods per hour, ~8000 mm3 of bacteria would accumulate in the effluent channel in 6 months.

    Lydia continued working with the microcontroller so that it sends an oscillating signal to the micropump. Bruce suggested that she read the manual first to figure out which pins on the chip are best to relay the signals. Kelvin finished the server and started working on the server to client transaction.

    #solar panel #filtration #electronics #software
    << Previous Week
    Next Week >>

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