Team:Penn State/Bidirectional Promoters Results

From 2012.igem.org

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     <p>Several BioBrick promoters have been tested in the bidirectional promoter construct. All successful ligations were cultured and the fluorescence of each individual promoter was tested in a TECAN machine.</P>
     <p>Several BioBrick promoters have been tested in the bidirectional promoter construct. All successful ligations were cultured and the fluorescence of each individual promoter was tested in a TECAN machine.</P>
<p>The following promoters have been successfully tested for quantitative directionality using the TECAN. For a <a href="https://2012.igem.org/Team:Penn_State/Parts">complete list</a> of promoters we intend to test, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a> webpage.</p>
<p>The following promoters have been successfully tested for quantitative directionality using the TECAN. For a <a href="https://2012.igem.org/Team:Penn_State/Parts">complete list</a> of promoters we intend to test, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a> webpage.</p>
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<p>Data collection and results are demonstrated graphically below</p>
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<p>Data collection and results are demonstrated graphically below for J23102, and three custom palindrome promoters. (Click on the graphs to see a larger version of the graph.)</p>
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<p>
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GFP Expression of all samples: <br/>
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<a href="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg">
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<img src="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg"/ width="500px" height="240px">
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</a>
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</p>
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<p>
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Average GFP Expression of all samples: <br/>
 +
<a href="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg">
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<img src="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg" width="300px" height="180px">
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</a>
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</p>
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<p>
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RFP Expression of all samples: <br/>
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<a href="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg">
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<img src="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg" width="500px" height="240px">
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</a>
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</p>
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<p>
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Average RFP Expression of all samples: <br/>
 +
<a href="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg">
 +
<img src="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg" width="300px" height="180px">
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</a>
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</p>
</div>
</div>
   </div>
   </div>

Revision as of 02:59, 4 October 2012

Bidirectional Promoters Overview

Bidirectional Promoters

Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce the expected proteins. Some bidirectional promoters are known to exist, but which way they promote and the degree of expression has not been quantified. This project will test the directionality of several BioBrick promoters to answer these questions.

Bidirectional Promoters

Sample navigation menu:

Overview | Design | Results

Data Collection

Several BioBrick promoters have been tested in the bidirectional promoter construct. All successful ligations were cultured and the fluorescence of each individual promoter was tested in a TECAN machine.

The following promoters have been successfully tested for quantitative directionality using the TECAN. For a complete list of promoters we intend to test, please visit our Parts webpage.

Data collection and results are demonstrated graphically below for J23102, and three custom palindrome promoters. (Click on the graphs to see a larger version of the graph.)

GFP Expression of all samples:

Average GFP Expression of all samples:

RFP Expression of all samples:

Average RFP Expression of all samples:

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