Team:Michigan/Notebook/Protocols
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+ | <div id="basic"> | ||
+ | <h3> | ||
+ | Protocols | ||
+ | </h3> | ||
+ | <h2>General</h2> | ||
+ | Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html> | ||
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+ | <h2>Cloning</h2> | ||
- | [ | + | Removing BioBricks from the Registry</html>[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]<html> |
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- | [[ | + | <br><br> |
- | + | Production of Competent Cells</html>[https://static.igem.org/mediawiki/2012/6/61/MichComp.pdf]<html> | |
+ | <br><br> | ||
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+ | Heat Shock Transformation</html>[https://static.igem.org/mediawiki/2012/0/0e/MichHeat.pdf]<html> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | DNA Purification: PCR</html>[https://static.igem.org/mediawiki/2012/0/04/MichPur2.pdf]<html> | ||
+ | DNA Purification: MiniPrep</html>[https://static.igem.org/mediawiki/2012/1/19/MichPur1.pdf]<html> | ||
+ | <br><br> | ||
+ | |||
+ | Restriction Enzyme Digest</html>[https://static.igem.org/mediawiki/2012/0/0f/MichRestrict.pdf]<html> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | Ligation</html>[https://static.igem.org/mediawiki/2012/1/10/MichLig.pdf]<html> | ||
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+ | <br><br> | ||
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+ | PCR (Phusion)</html>[https://static.igem.org/mediawiki/2012/9/94/MichPCR.pdf]<html> | ||
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+ | <br><br> | ||
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+ | Colony PCR</html>[https://static.igem.org/mediawiki/2012/9/96/MichCol.pdf]<html> | ||
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+ | <br><br> | ||
+ | |||
+ | Gel Electrophoresis</html>[https://static.igem.org/mediawiki/2012/1/12/MichElectro.pdf]<html> | ||
+ | |||
+ | <br><br> | ||
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+ | <h2>Characterization</h2> | ||
+ | <br> | ||
+ | Transmission Electron Microscopy Protocol</html>[https://static.igem.org/mediawiki/2012/0/0a/MichTEM.pdf]<html> | ||
+ | <br><br> | ||
+ | FimE Asymmetrical Digest Assay Protocol</html>[https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf]<html> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </html> |
Latest revision as of 02:26, 4 October 2012
Protocols
General
Plate Production[1]Cloning
Removing BioBricks from the Registry[2]Production of Competent Cells[3]
Heat Shock Transformation[4]
DNA Purification: PCR[5] DNA Purification: MiniPrep[6]
Restriction Enzyme Digest[7]
Ligation[8]
PCR (Phusion)[9]
Colony PCR[10]
Gel Electrophoresis[11]
Characterization
Transmission Electron Microscopy Protocol[12]
FimE Asymmetrical Digest Assay Protocol[13]